Click image to see more details
Product Info Summary
| SKU: | M00101-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-IL1 beta Rabbit Monoclonal Antibody
View all IL-1 beta/IL-1F2 Antibodies
SKU/Catalog Number
M00101-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-IL1 beta Rabbit Monoclonal Antibody catalog # M00101-1. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00101-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BII-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human IL1 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00101-1 is reactive to IL1B in Human
Applications
M00101-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
21 kDa
Calculated molecular weight
30.748kDa
Background of IL-1 beta/IL-1F2
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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Western blot analysis of extracts from recombinant IL1 beta, using IL1 beta antibody.
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30.748kDa
Superfamily
IL-1 family
Alternative Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL1 beta Rabbit Monoclonal Antibody (M00101-1)
Hello CJ!
M00101-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis,Life Sciences,Volume 226,2019,Pages 164-172,ISSN 0024-3205,https://doi.org/10.1016/j. lfs.2019.03.071.
Species: Human
M00101-1 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA
Quantitative proteomic analysis of host responses triggered by Mycobacterium tuberculosis infection in human macrophage cells
Antenatal exposure of maternal secondhand smoke (SHS) increases fetal lung expression of RAGE and induces RAGE-mediated pulmonary inflammation
NLRP3 Inflammasome Sequential Changes in?Staphylococcus aureus-Induced Mouse Model of Acute Rhinosinusitis
Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Int J Mol Sci. 2012;13(7):8379-87. Doi: 10.3390/Ijms13078379. Epub 2012 Jul 5. Astragalus Membranaceus Inhibits Inflammation Via Phospho-P38 Mitogen-Activated Protein Kinase (Mapk) And Nuclear Factor (Nf)-...
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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14 Customer Q&As for Anti-IL1 beta Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-IL1 beta Rabbit Monoclonal antibody M00101-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-06
Question
Please see the WB image, lot number and protocol we used for leukocyte using anti-IL1 beta Rabbit Monoclonal antibody M00101-1. Please let me know if you require anything else.
P. Wu
Verified customer
Asked: 2020-03-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-10
Question
My question regarding product M00101-1, anti-IL1 beta Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00101-1 anti-IL1 beta Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-03
Question
My boss were happy with the WB result of your anti-IL1 beta Rabbit Monoclonal antibody. However we have been able to see positive staining in smooth muscle tissue cytoplasm using this antibody. Is that expected? Could you tell me where is IL1B supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
According to literature, smooth muscle tissue does express IL1B. Generally IL1B expresses in cytoplasm, cytosol. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2019-12-11
Question
Is this M00101-1 anti-IL1 beta Rabbit Monoclonal antibody reactive to the isotypes of IL1B?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The immunogen of M00101-1 anti-IL1 beta Rabbit Monoclonal antibody is A synthesized peptide derived from human IL1 beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-22
Question
Does M00101-1 anti-IL1 beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
You can see on the product datasheet, M00101-1 anti-IL1 beta Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-15
Question
you antibody to test anti-IL1 beta Rabbit Monoclonal antibody M00101-1 on human leukocyte for research purposes, then I may be interested in using anti-IL1 beta Rabbit Monoclonal antibody M00101-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The products we sell, including anti-IL1 beta Rabbit Monoclonal antibody M00101-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-23
Question
Would anti-IL1 beta Rabbit Monoclonal antibody M00101-1 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
According to the expression profile of leukocyte, IL1B is highly expressed in leukocyte. So, it is likely that anti-IL1 beta Rabbit Monoclonal antibody M00101-1 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2018-04-13
Question
I see that the anti-IL1 beta Rabbit Monoclonal antibody M00101-1 works with WB, what is the protocol used to produce the result images on the product page?
A. Taylor
Verified customer
Asked: 2018-02-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-02-05
Question
We are currently using anti-IL1 beta Rabbit Monoclonal antibody M00101-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Z. Lewis
Verified customer
Asked: 2016-06-06
Answer
The anti-IL1 beta Rabbit Monoclonal antibody (M00101-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-06-06
Question
Do you have a BSA free version of anti-IL1 beta Rabbit Monoclonal antibody M00101-1 available?
A. Patel
Verified customer
Asked: 2016-05-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL1 beta Rabbit Monoclonal antibody M00101-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-05-16
Question
Is a blocking peptide available for product anti-IL1 beta Rabbit Monoclonal antibody (M00101-1)?
C. Lewis
Verified customer
Asked: 2015-12-17
Answer
We do provide the blocking peptide for product anti-IL1 beta Rabbit Monoclonal antibody (M00101-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-12-17
Question
We have observed staining in human skin. Are there any suggestions? Is anti-IL1 beta Rabbit Monoclonal antibody supposed to stain skin positively?
C. Anderson
Verified customer
Asked: 2014-10-03
Answer
According to literature skin does express IL1B. According to Uniprot.org, IL1B is expressed in smooth muscle tissue, leukocyte, histiocytic lymphoma, monocyte, lung, skin, macrophage, among other tissues. Regarding which tissues have IL1B expression, here are a few articles citing expression in various tissues:
Histiocytic lymphoma, Pubmed ID: 3493774
Leukocyte, Pubmed ID: 3490654
Lung, Pubmed ID: 15489334
Macrophage, Pubmed ID: 20148899
Monocyte, Pubmed ID: 2635664, 11991722
Skin, Pubmed ID: 1919436
Boster Scientific Support
Answered: 2014-10-03
Question
I was wanting to use your anti-IL1 beta Rabbit Monoclonal antibody for WB for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
B. Krishna
Verified customer
Asked: 2013-03-18
Answer
As indicated on the product datasheet, M00101-1 anti-IL1 beta Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-18
