Product Info Summary
SKU: | M01247-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Monkey, Mouse |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IRAK-4 Monoclonal Antibody
SKU/Catalog Number
M01247-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-IRAK-4 Monoclonal Antibody catalog # M01247-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IRAK-4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01247-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2H9
Isotype
IgG
Immunogen
Purified recombinant fragment of human IRAK-4 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01247-1 is reactive to IRAK4 in Human, Monkey, Mouse
Applications
M01247-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
51.53kDa
Background of IRAK4
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. ELISA validation of IRAK4 using Anti-IRAK-4 Monoclonal Antibody (M01247-1).
ELISA analysis of IRAK-4 antibody.Click image to see more details
Figure 3. Flow Cytometry validation of IRAK4 using Anti-IRAK-4 Monoclonal Antibody (M01247-1).
Flow cytometric (FCM) analysis of HeLa cells using IRAK-4 Monoclonal Antibody (blue) and negative control (red).Click image to see more details
Figure 2. Immunohistochemistry validation of IRAK4 using Anti-IRAK-4 Monoclonal Antibody (M01247-1).
Immunohistochemistry (IHC) analysis of paraffin-embedded human lung cancer Tissues with DAB staining using IRAK-4 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-IRAK-4 Monoclonal Antibody M01247-1
Western Blot (WB) analysis using IRAK-4 Monoclonal Antibody against THP-1 (1)Protein Target Info & Infographic
Gene/Protein Information For IRAK4 (Source: Uniprot.org, NCBI)
Gene Name
IRAK4
Full Name
Interleukin-1 receptor-associated kinase 4
Weight
51.53kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11; EC 2.7.11.1; interleukin-1 receptor-associated kinase 4; IPD1; IRAK4; IRAK-4; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64 IRAK4 IMD67, IPD1, IRAK-4, NY-REN-64, REN64 interleukin 1 receptor associated kinase 4 interleukin-1 receptor-associated kinase 4|renal carcinoma antigen NY-REN-64
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IRAK4, check out the IRAK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IRAK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IRAK-4 Monoclonal Antibody (M01247-1)
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No publications found for M01247-1
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Customer Reviews
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