Anti-Methyl-CpG-binding protein 2 MeCP2 Antibody

Product Name

Anti-Methyl-CpG-binding protein 2 MeCP2 Antibody

View all MECP2 Antibodies

SKU/Catalog Number

CI1047

Size

50μg/42μl

Form

Liquid

Description

Boster Bio Anti-Methyl-CpG-binding protein 2 MeCP2 Antibody (Catalog# CI1047). Tested in ChIP, ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.

Cite This Product

Anti-Methyl-CpG-binding protein 2 MeCP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1047)

Host

Rabbit

Contents

Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Clonality

Polyclonal

Immunogen

This antibody is raised in rabbit against MeCP2 (Methyl-CpG-binding domain protein 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

CI1047 is reactive to MECP2 in Human

Applications

CI1047 is guaranteed for ChIP, ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

52.441kDa

Background of MECP2

MeCP2 is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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ChIP assays were performed using human osteosarcoma (U2OS) cells, Anti-Suz12 polyclonal antibody (Catalog # CI1047) and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 5 μg of antibody were used per ChIP experiment. IgG (1 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoter of the ZMYND10 gene, used as a positive control and for the promoter of the CDC6 gene, used as a negative control.

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To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-MeCP2 polyclonal antibody (Catalog # CI1047) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.

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Western blot analysis of MeCP2 expression in nuclear extracts from HeLa cells (40

Gene/Protein Information For MECP2 (Source: Uniprot.org, NCBI)

Alternative Names

AUTSX3; DKFZp686A24160; MeCp-2 protein; mental retardation, X-linked 79; methyl CpG binding protein 2 (Rett syndrome); X-linked 16 MECP2 AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT methyl-CpG binding protein 2 methyl-CpG-binding protein 2|meCp-2 protein

For more info on MECP2, check out the MECP2 Infographic

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In this infographic, you will see the following information for MECP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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