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Product Info Summary
| SKU: | M00047-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MeCP2 Antibody
SKU/Catalog Number
M00047-1
Size
100ul
Description
Boster Bio Anti-MeCP2 Antibody catalog # M00047-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MeCP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00047-1)
Host
Rabbit
Contents
Affinity purified at 1mg/mL in PBS, 50% glycerol, 5mM NaN3
Clonality
Polyclonal
Immunogen
Full length recombinant human MeCP2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00047-1 is reactive to MECP2 in Human, Monkey, Mouse, Rat
Applications
M00047-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
52.441kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:10,000-1:20,000 IF/ICC or IHC: 1:2,000
Validation Images & Assay Conditions
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Western blot analysis of tissue and cell lysates using rabbit pAb to MeCP2, M00047-1, dilution 1:20,000 in green: [1] protein standard (red), [2] Rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain, [6] HeLa cells, [7] C6 cells. Major band at about 70-75 kDa corresponds to MeCP2 protein, predominantly detected in the nuclear fraction of the lysates. The apparent molecular weights of MeCP2 based on SDS-PAGE mobility are variable between species and higher than the actual MeCP2 molecular weight which is ~54kDa
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Immunofluorescent analysis of rat cerebellum section costained with rabbit pAb to MeCP2, M00047-1, dilution 1:5,000, in red, and chicken pAb to calbindin, dilution 1:2,000, in green. Blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 M, and free-floating sections were stained with above antibodies. The MeCP2 antibody selectively stains nuclei of neuronal cells. Calbindin, often used as Purkinje cell marker, is prominently expressed in dendrites and perikarya of these cells in the cerebellar molecular layer.
Protein Target Info & Infographic
Gene/Protein Information For MECP2 (Source: Uniprot.org, NCBI)
Gene Name
MECP2
Full Name
Methyl-CpG-binding protein 2
Weight
52.441kDa
Alternative Names
AUTSX3; DKFZp686A24160; MeCp-2 protein; mental retardation, X-linked 79; methyl CpG binding protein 2 (Rett syndrome); X-linked 16 MECP2 AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT methyl-CpG binding protein 2 methyl-CpG-binding protein 2|meCp-2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MECP2, check out the MECP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MECP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MeCP2 Antibody (M00047-1)
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No publications found for M00047-1
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-MeCP2 Antibody
Question
I was wanting to use to test anti-MeCP2 antibody M00047-1 on human t-cell for research purposes, then I may be interested in using anti-MeCP2 antibody M00047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
The products we sell, including anti-MeCP2 antibody M00047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-24
Question
Is a blocking peptide available for product anti-MeCP2 antibody (M00047-1)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
We do provide the blocking peptide for product anti-MeCP2 antibody (M00047-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-26
Question
Will M00047-1 anti-MeCP2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
It shows on the product datasheet, M00047-1 anti-MeCP2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-27
Question
We have observed staining in rat cervix carcinoma erythroleukemia. What should we do? Is anti-MeCP2 antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express MECP2. From what I have seen in Uniprot.org, MECP2 is expressed in cerebellar vermis, placenta, colon endothelium, skeletal muscle, cervix carcinoma, t-cell, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have MECP2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon endothelium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Skeletal muscle, Pubmed ID: 8672133
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2019-08-05
Question
Have you ever done immunostaining with M00047-1?
Verified customer
Asked: 2019-08-01
Answer
Unfortunately, we have not done any in-house immunostaining with Anti-MeCP2 Antibody (M00047-1).
Boster Scientific Support
Answered: 2019-08-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-MeCP2 antibody M00047-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-02
Question
I have attached the WB image, lot number and protocol we used for t-cell using anti-MeCP2 antibody M00047-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
We are currently using anti-MeCP2 antibody M00047-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
The anti-MeCP2 antibody (M00047-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-07
Question
Will anti-MeCP2 antibody M00047-1 work for IHC with t-cell?
M. Taylor
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of t-cell, MECP2 is highly expressed in t-cell. So, it is likely that anti-MeCP2 antibody M00047-1 will work for IHC with t-cell.
Boster Scientific Support
Answered: 2018-11-14
Question
We ordered your anti-MeCP2 antibody for WB on cervix carcinoma erythroleukemia last year. I am using rat, and We are going to use the antibody for IHC next. My lab would like examining cervix carcinoma erythroleukemia as well as skeletal muscle in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
M. Johnson
Verified customer
Asked: 2018-10-11
Answer
I took a look at the website and datasheets of our anti-MeCP2 antibody and it seems that M00047-1 has been tested on rat in both WB and IHC. Thus M00047-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-10-11
Question
Our team were content with the WB result of your anti-MeCP2 antibody. However we have observed positive staining in skeletal muscle nucleus. using this antibody. Is that expected? Could you tell me where is MECP2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
According to literature, skeletal muscle does express MECP2. Generally MECP2 expresses in nucleus. Regarding which tissues have MECP2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon endothelium, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 15489334
Skeletal muscle, Pubmed ID: 8672133
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-06-29
Question
I was wanting to use your anti-MeCP2 antibody for IHC for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
J. Lewis
Verified customer
Asked: 2018-05-15
Answer
As indicated on the product datasheet, M00047-1 anti-MeCP2 antibody has been tested for IHC, WB on human, monkey, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-15
Question
Is this M00047-1 anti-MeCP2 antibody reactive to the isotypes of MECP2?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
The immunogen of M00047-1 anti-MeCP2 antibody is Full length recombinant human MeCP2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-03-06
Question
Is there a BSA free version of anti-MeCP2 antibody M00047-1 available?
T. Evans
Verified customer
Asked: 2017-07-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MeCP2 antibody M00047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-21
Question
I see that the anti-MeCP2 antibody M00047-1 works with IHC, what is the protocol used to produce the result images on the product page?
J. Roberts
Verified customer
Asked: 2017-04-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-04-21
Question
My question regarding product M00047-1, anti-MeCP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Li
Verified customer
Asked: 2016-12-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00047-1 anti-MeCP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-12-07
Question
I am interested in using your anti-MeCP2 antibody for phosphatidylcholine metabolic process studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
L. Rodriguez
Verified customer
Asked: 2013-12-19
Answer
We appreciate your inquiry. This M00047-1 anti-MeCP2 antibody is tested on mouse brain, hela cells, c6 cells, neuronal cells. It is guaranteed to work for IHC, WB in human, monkey, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-12-19
