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Product Info Summary
| SKU: | M01488 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Met (c-Met) Rabbit Monoclonal Antibody
View all HGFR/c-MET Antibodies
SKU/Catalog Number
M01488
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Met (c-Met) Rabbit Monoclonal Antibody catalog # M01488. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Met (c-Met) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01488)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BFA-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Met (c-Met)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01488 is reactive to MET in Human, Mouse, Rat
Applications
M01488 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
155.541kDa
Background of HGFR/c-MET
Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of HT-29 cells, using Met (c-Met) Antibody .
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Western blot analysis of c-Met expression in 293 cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human stomach, using Met (c-Met) Antibody .
Protein Target Info & Infographic
Gene/Protein Information For MET (Source: Uniprot.org, NCBI)
Gene Name
MET
Full Name
Hepatocyte growth factor receptor
Weight
155.541kDa
Superfamily
protein kinase superfamily
Alternative Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MET, check out the MET Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MET: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Met (c-Met) Rabbit Monoclonal Antibody (M01488)
Hello CJ!
M01488 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen X, Ding G, Gao Q, Sun J, Zhang Q, Du L, Qiu Z, Wang C, Zheng F, Sun B, Ni J, Feng Z, Zhu J. Plos One. 2013 May 13;8(5):E63093. Doi: 10.1371/Journal.Pone.0063093. Print 2013. A Human Anti-C-Met Fab Fragment Conjugated With Doxorubicin As Targe...
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-Met (c-Met) Rabbit Monoclonal Antibody
Question
Is it possible to detect human c-Met using M01488?
Verified customer
Asked: 2021-02-23
Answer
Yes, the Anti-Met (C-Met) Rabbit Monoclonal Antibody (M01488) is able to detect human c-Met. The immunogen for M01488 is CKEALAKSEMNVNMKYQLP (Uniprot ID:P08581).
Boster Scientific Support
Answered: 2021-02-24
Question
Is this M01488 anti-Met (c-Met) Rabbit Monoclonal antibody reactive to the isotypes of MET?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
The immunogen of M01488 anti-Met (c-Met) Rabbit Monoclonal antibody is A synthesized peptide derived from human Met (c-Met) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-28
Question
I was wanting to use to test anti-Met (c-Met) Rabbit Monoclonal antibody M01488 on human hepatoma for research purposes, then I may be interested in using anti-Met (c-Met) Rabbit Monoclonal antibody M01488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The products we sell, including anti-Met (c-Met) Rabbit Monoclonal antibody M01488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-14
Question
Will anti-Met (c-Met) Rabbit Monoclonal antibody M01488 work for ICC with hepatoma?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of hepatoma, MET is highly expressed in hepatoma. So, it is likely that anti-Met (c-Met) Rabbit Monoclonal antibody M01488 will work for ICC with hepatoma.
Boster Scientific Support
Answered: 2019-10-24
Question
Will anti-Met (c-Met) Rabbit Monoclonal antibody M01488 work on bovine IHC with pigmented layer of retina?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
Our lab technicians have not validated anti-Met (c-Met) Rabbit Monoclonal antibody M01488 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Met (c-Met) Rabbit Monoclonal antibody M01488 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine pigmented layer of retina in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-26
Question
I have a question about product M01488, anti-Met (c-Met) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01488 anti-Met (c-Met) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-31
Question
Please see the WB image, lot number and protocol we used for hepatoma using anti-Met (c-Met) Rabbit Monoclonal antibody M01488. Please let me know if you require anything else.
J. Kulkarni
Verified customer
Asked: 2018-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-28
Question
Is there a BSA free version of anti-Met (c-Met) Rabbit Monoclonal antibody M01488 available?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Met (c-Met) Rabbit Monoclonal antibody M01488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-14
