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Product Info Summary
| SKU: | M00194-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MHC class I HLA-A Rabbit Monoclonal Antibody
SKU/Catalog Number
M00194-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MHC class I HLA-A Rabbit Monoclonal Antibody catalog # M00194-2. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MHC class I HLA-A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00194-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOID-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MHC class I
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00194-2 is reactive to HLA-A in Human
Applications
M00194-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
40.841kDa
Background of HLA-A
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of MHC class I expression in Raji cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For HLA-A (Source: Uniprot.org, NCBI)
Gene Name
HLA-A
Full Name
HLA class I histocompatibility antigen, A alpha chain
Weight
40.841kDa
Superfamily
MHC class I family
Alternative Names
A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLAA; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80 HLA-A HLAA major histocompatibility complex, class I, A HLA class I histocompatibility antigen, A alpha chain|HLA class I histocompatibility antigen, A-1 alpha chain|MHC class I antigen HLA-A heavy chain|leukocyte antigen class I-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-A, check out the HLA-A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MHC class I HLA-A Rabbit Monoclonal Antibody (M00194-2)
Hello CJ!
No publications found for M00194-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-MHC class I HLA-A Rabbit Monoclonal Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-MHC class I Rabbit Monoclonal antibody M00194-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-03
Question
We are currently using anti-MHC class I Rabbit Monoclonal antibody M00194-2 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-MHC class I Rabbit Monoclonal antibody (M00194-2) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
We want to test anti-MHC class I Rabbit Monoclonal antibody M00194-2 on human cervix carcinoma for research purposes, then I may be interested in using anti-MHC class I Rabbit Monoclonal antibody M00194-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
The products we sell, including anti-MHC class I Rabbit Monoclonal antibody M00194-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-09
Question
Is this M00194-2 anti-MHC class I Rabbit Monoclonal antibody reactive to the isotypes of HLA-A?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The immunogen of M00194-2 anti-MHC class I Rabbit Monoclonal antibody is A synthesized peptide derived from human MHC class I. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-31
Question
I see that the anti-MHC class I Rabbit Monoclonal antibody M00194-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-26
Question
We have seen staining in human brain. Any tips? Is anti-MHC class I Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
From literature brain does express HLA-A. From Uniprot.org, HLA-A is expressed in blood, brain, liver, cervix carcinoma, among other tissues. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2017-08-09
Question
I was wanting to use your anti-MHC class I Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-05-31
Answer
You can see on the product datasheet, M00194-2 anti-MHC class I Rabbit Monoclonal antibody has been tested for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-31
Question
Is a blocking peptide available for product anti-MHC class I Rabbit Monoclonal antibody (M00194-2)?
E. Walker
Verified customer
Asked: 2016-02-23
Answer
We do provide the blocking peptide for product anti-MHC class I Rabbit Monoclonal antibody (M00194-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-02-23
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-MHC class I Rabbit Monoclonal antibody M00194-2. Please let me know if you require anything else.
D. Evans
Verified customer
Asked: 2015-04-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-30
Question
We have tried in the past anti-MHC class I Rabbit Monoclonal antibody for WB on liver a few years ago. I am using human, and I plan to use the antibody for IF next. My question regards examining liver as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
P. Jha
Verified customer
Asked: 2015-04-28
Answer
I have checked the website and datasheets of our anti-MHC class I Rabbit Monoclonal antibody and it appears that M00194-2 has been validated on human in both WB and IF. Thus M00194-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-04-28
Question
Will anti-MHC class I Rabbit Monoclonal antibody M00194-2 work for IF with cervix carcinoma?
A. Jackson
Verified customer
Asked: 2015-03-16
Answer
According to the expression profile of cervix carcinoma, HLA-A is highly expressed in cervix carcinoma. So, it is likely that anti-MHC class I Rabbit Monoclonal antibody M00194-2 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2015-03-16
Question
Will M00194-2 anti-MHC class I Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Gonzalez
Verified customer
Asked: 2015-02-18
Answer
As indicated on the product datasheet, M00194-2 anti-MHC class I Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-02-18
Question
Our team were satisfied with the WB result of your anti-MHC class I Rabbit Monoclonal antibody. However we have been able to see positive staining in liver cell membrane using this antibody. Is that expected? Could you tell me where is HLA-A supposed to be expressed?
H. Li
Verified customer
Asked: 2014-09-15
Answer
From what I have seen in literature, liver does express HLA-A. Generally HLA-A expresses in cell membrane. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2014-09-15
Question
Is there a BSA free version of anti-MHC class I Rabbit Monoclonal antibody M00194-2 available?
C. Wu
Verified customer
Asked: 2014-01-30
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MHC class I Rabbit Monoclonal antibody M00194-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-01-30
Question
My question regarding product M00194-2, anti-MHC class I Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Mangal
Verified customer
Asked: 2013-01-08
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00194-2 anti-MHC class I Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-08
