Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated

Product Name

Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated

View all CCL20/MIP-3 alpha Antibodies

SKU/Catalog Number

B00748-1

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated (Catalog # B00748-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store MIP-3 alpha Antibody Biotin Conjugated at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-MIP-3 alpha Ccl20 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00748-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

MIP-3 alpha Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-3α protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

B00748-1 is reactive to CCL20 in Human

Applications

B00748-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

10.762kDa

Background of CCL20/MIP-3 alpha

MIP-3α (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3α inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3α also possesses antibacterial activity against E.coli and S.aureus. MIP-3α is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. MIP-1 alpha Antibody Biotin Conjugated is useful for researchers interested in Cancer, Cell Biology and Immunology Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000

Validation Images & Assay Conditions

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Western Blot of Biotin Conjugated Rabbit Anti-Human MIP-3 alpha polyclonal antibody (Catalog # B00748-1) at 1:1000. Lane 1: Human MIP-3 alpha. Lane 2: None. Load: 50 ng per lane. Predicted/Observed size: 10 kDa, 10 kDa for Human MIP-3 alpha. Other band(s): None. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For CCL20 (Source: Uniprot.org, NCBI)

Alternative Names

beta chemokine exodus-1; Beta-chemokine exodus-1; CC chemokine LARC; C-C motif chemokine 20; CCL20; chemokine (C-C motif) ligand 20; CKb4; exodus-1; LARC; LARCLiver and activation-regulated chemokine; MIP3 alpha; MIP-3 alpha; MIP-3a; MIP-3-alpha; MIP3AMacrophage inflammatory protein 3 alpha; SCYA20Small-inducible cytokine A20; small inducible cytokine subfamily A (Cys-Cys), member 20; ST38 CCL20 CKb4, Exodus, LARC, MIP-3-alpha, MIP-3a, MIP3A, SCYA20, ST38 C-C motif chemokine ligand 20 C-C motif chemokine 20|CC chemokine LARC|beta chemokine exodus-1|chemokine (C-C motif) ligand 20|liver and activation-regulated chemokine|macrophage inflammatory protein 3 alpha|small inducible cytokine subfamily A (Cys-Cys), member 20|small-inducible cytokine A20

For more info on CCL20, check out the CCL20 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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