Anti-Mitofusin 1/MFN1 Antibody Picoband™

Product Name

Anti-Mitofusin 1/MFN1 Antibody Picoband™

View all Mitofusin 1 Antibodies

SKU/Catalog Number

PB9264

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mitofusin 1/MFN1 Antibody Picoband™ catalog # PB9264. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mitofusin 1/MFN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9264)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminal of human Mitofusin 1, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9264 is reactive to MFN1 in Human, Mouse, Rat

Applications

PB9264 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

84 kDa

Calculated molecular weight

84.16kDa

Background of Mitofusin 1

Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene. It is an 80­90 kDa mitochondrial member of the dynamin family of molecules. It is ubiquitously expressed, and found in the outer mitochondrial membrane. The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology. MFN1 is known to form oligomers, either with itself or MFN­2, and to undergo ubiquitination by MARCH5. MFN­1 has two key domains. One is a coiled­coil region that mediates MFN­1: MFN­1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion. Overexpression of MFN1 caused perinuclear mitochondrial clustering.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Mitofusin 1 using anti-Mitofusin 1 antibody (PB9264).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: mouse heart tissue lysates,
Lane 5: human placenta tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofusin 1 antigen affinity purified polyclonal antibody (Catalog # PB9264) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mitofusin 1 at approximately 84KD. The expected band size for Mitofusin 1 is at 84KD.

Gene/Protein Information For MFN1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp762F247; EC 3.6.5; EC 3.6.5.-; FLJ20693; Fzo homolog; hfzo1; hfzo2; MFN1; MGC41806; mitochondrial transmembrane GTPase Fzo-1; mitochondrial transmembrane GTPase FZO-2; Mitofusin 1; mitofusin-1; putative transmembrane GTPase; Transmembrane GTPase MFN1 MFN1 hfzo1, hfzo2 mitofusin 1 mitofusin-1|fzo homolog|mitochondrial transmembrane GTPase FZO-2|mitochondrial transmembrane GTPase Fzo-1|putative transmembrane GTPase|transmembrane GTPase MFN1

For more info on MFN1, check out the MFN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MFN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MFN1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis