Click image to see more details
Product Info Summary
| SKU: | M00656 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
View all MMP-14/MT1-MMP Antibodies
SKU/Catalog Number
M00656
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody catalog # M00656. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00656)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BFD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP14
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00656 is reactive to MMP14 in Human, Mouse, Rat
Applications
M00656 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
65.894kDa
Background of MMP-14/MT1-MMP
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of MMP14 expression in human spleen lysate.
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Immunohistochemical analysis of paraffin-embedded human kdieny, using MMP14 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For MMP14 (Source: Uniprot.org, NCBI)
Gene Name
MMP14
Full Name
Matrix metalloproteinase-14
Weight
65.894kDa
Superfamily
peptidase M10A family
Alternative Names
EC 3.4.24; EC 3.4.24.80; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane-inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT1-MMPMTMMP1; MT-MMP 1; MT-MMP1 MMP14 MMP-14, MMP-X1, MT-MMP, MT-MMP 1, MT1-MMP, MT1MMP, MTMMP1, WNCHRS matrix metallopeptidase 14 matrix metalloproteinase-14|matrix metallopeptidase 14 (membrane-inserted)|membrane type 1 metalloprotease|membrane-type-1 matrix metalloproteinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP14, check out the MMP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody (M00656)
Hello CJ!
M00656 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-?1 pathway
Zhang Jt, Sun W, Zhang Wz, Ge Cy, Liu Zy, Zhao Zm, Lu Xs, Fan Yz. Bmc Cancer. 2014 Mar 15;14:193. Doi: 10.1186/1471-2407-14-193. Norcantharidin Inhibits Tumor Growth And Vasculogenic Mimicry Of Human Gallbladder Carcinomas By Suppression Of The Pi...
Zhou H, Wu A, Fu W, Lv Z, Zhang Z. Oncol Lett. 2014 May;7(5):1395-1400. Epub 2014 Feb 27. Significance Of Semaphorin-3A And Mmp-14 Protein Expression In Non-Small Cell Lung Cancer.
Xiao H, Li H, Yu G, Xiao W, Hu J, Tang K, Zeng J, He W, Zeng G, Ye Z, Xu H. Oncol Rep. 2014 Apr;31(4):1832-8. Doi: 10.3892/Or.2014.3048. Epub 2014 Feb 24. Microrna-10B Promotes Migration And Invasion Through Klf4 And Hoxd10 In Human Bladder Cancer.
Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-MMP14/Mt1 Mmp Rabbit Monoclonal Antibody
Question
Will anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 work for IHC with skin?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
According to the expression profile of skin, MMP14 is highly expressed in skin. So, it is likely that anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 will work for IHC with skin.
Boster Scientific Support
Answered: 2020-04-28
Question
I see that the anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-30
Question
Is a blocking peptide available for product anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656)?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
We do provide the blocking peptide for product anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-18
Question
I have a question about product M00656, anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for skin using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
Do you have a BSA free version of anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 available?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-27
Question
We have seen staining in mouse lung. Any tips? Is anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody supposed to stain lung positively?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
Based on literature lung does express MMP14. Based on Uniprot.org, MMP14 is expressed in endocervix, placenta, lung, tongue, skin, melanoma, among other tissues. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-06
Question
Is this M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody reactive to the isotypes of MMP14?
S. Brown
Verified customer
Asked: 2018-09-24
Answer
The immunogen of M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP14 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-24
Question
See below the WB image, lot number and protocol we used for skin using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656. Please let me know if you require anything else.
P. Johnson
Verified customer
Asked: 2018-08-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-27
Question
Would anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 work on feline IF with placenta?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
Our lab technicians have not tested anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 on feline. You can run a BLAST between feline and the immunogen sequence of anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline placenta in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-02
Question
We are interested in to test anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 on human skin for research purposes, then I may be interested in using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
The products we sell, including anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-02-23
Question
Will M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Roberts
Verified customer
Asked: 2017-07-20
Answer
It shows on the product datasheet, M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-20
Question
We are currently using anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody M00656 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
J. Jones
Verified customer
Asked: 2017-04-13
Answer
The anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody (M00656) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-13
Question
Our lab were well pleased with the WB result of your anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody. However we have seen positive staining in endocervix membrane using this antibody. Is that expected? Could you tell me where is MMP14 supposed to be expressed?
O. Wu
Verified customer
Asked: 2015-05-07
Answer
From what I have seen in literature, endocervix does express MMP14. Generally MMP14 expresses in membrane. Regarding which tissues have MMP14 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 7649159
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 7721107, 8015608
Skin, Pubmed ID: 15489334
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-05-07
Question
We have tried in the past anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody for IP on tongue in the past. I am using human, and We are going to use the antibody for IHC next. you antibody examining tongue as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
S. Dhar
Verified customer
Asked: 2014-07-24
Answer
I viewed the website and datasheets of our anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody and it seems that M00656 has been validated on human in both IP and IHC. Thus M00656 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-07-24
Question
I was wanting to use your anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody for IHC for human skin on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human skin identification?
Z. Walker
Verified customer
Asked: 2013-09-18
Answer
As indicated on the product datasheet, M00656 anti-MMP14/Mt1 Mmp Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-18
