Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody

Product Name

Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody

View all N-Cadherin Antibodies

SKU/Catalog Number

M01577-1

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody catalog # M01577-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-N-Cadherin-2 CDH2 CD325-Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01577-1)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FI-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human N Cadherin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01577-1 is reactive to CDH2 in Human, Mouse, Rat

Applications

M01577-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

140 kDa

Calculated molecular weight

99.809kDa

Background of N-Cadherin

Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX (K/R) R consensus motif.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

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Immunohistochemical analysis of paraffin-embedded Rat testis, using the Antibody at 1:100 dilution.

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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (M01577-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified polyclonal antibody (Catalog # M01577-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140 kDa. The expected band size for CDH2 is at 100 kDa.

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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human colon cancer, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.

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Immunohistochemical analysis of paraffin-embedded human colon, using N Cadherin Antibody.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For CDH2 (Source: Uniprot.org, NCBI)

Alternative Names

cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin CDH2 ACOGS, ARVD14, CD325, CDHN, CDw325, NCAD cadherin 2 cadherin-2|N-cadherin 1|cadherin 2, type 1, N-cadherin (neuronal)|calcium-dependent adhesion protein, neuronal|neural cadherin

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