Product Info Summary
SKU: | PA2111-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-NFkB/NFKB1 p105/p50 Antibody
SKU/Catalog Number
PA2111-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFkB/NFKB1 p105/p50 Antibody catalog # PA2111-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB/NFKB1 p105/p50 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2111-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human NFkB/NFKB1 p105/p50, different from the related rat sequence by one amino acid and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2111-1 is reactive to NFKB1 in Human, Mouse, Rat
Applications
PA2111-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
105 kDa
Calculated molecular weight
105.356kDa
Background of NFKB1
Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-NFkB p105/p50 antibody, PA2111-1, Western blotting
All lanes: Anti NFkB p105/p50(PA2111-1) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 105KD
Observed bind size: 105KD
Protein Target Info & Infographic
Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)
Gene Name
NFKB1
Full Name
Nuclear factor NF-kappa-B p105 subunit
Weight
105.356kDa
Alternative Names
DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B; NF-kappabeta; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151; p105; p50 NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NFKB1, check out the NFKB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB/NFKB1 p105/p50 Antibody (PA2111-1)
Hello CJ!
PA2111-1 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
HMGB1, TGF-? and NF-?B are associated with chronic allograft nephropathy
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
Leng Am, Liu T, Yang J, Cui Jf, Li Xh, Zhu Yn, Xiong T, Zhang G, Chen Y. Cell Biol Int. 2012 Oct 1;36(10):893-9. Doi: 10.1042/Cbi20110473. The Apoptotic Effect And Associated Signalling Of Hsp90 Inhibitor 17-Dmag In Hepatocellular Carcinoma Cells.
Zhao Yj, Wang H, Liu X, Sun M, Kazuhiro H. Mol Med Rep. 2012 Oct;6(4):739-44. Doi: 10.3892/Mmr.2012.1007. Epub 2012 Jul 26. Protective Effects Of Glutamine In A Rat Model Of Endotoxemia.
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-NFkB/NFKB1 p105/p50 Antibody
Question
I see that the anti-NFkB p105/p50/NFKB1 antibody PA2111-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-16
Question
Does anti-NFkB p105/p50/NFKB1 antibody PA2111-1 work on pig WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
Our lab technicians have not validated anti-NFkB p105/p50/NFKB1 antibody PA2111-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-NFkB p105/p50/NFKB1 antibody PA2111-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-NFkB p105/p50/NFKB1 antibody PA2111-1. Let me know if you need anything else.
O. Parker
Verified customer
Asked: 2018-06-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-01
Question
Do you have a BSA free version of anti-NFkB p105/p50/NFKB1 antibody PA2111-1 available?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NFkB p105/p50/NFKB1 antibody PA2111-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-11
Question
I was wanting to use to test anti-NFkB p105/p50/NFKB1 antibody PA2111-1 on mouse liver for research purposes, then I may be interested in using anti-NFkB p105/p50/NFKB1 antibody PA2111-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Thomas
Verified customer
Asked: 2017-02-20
Answer
The products we sell, including anti-NFkB p105/p50/NFKB1 antibody PA2111-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-02-20
Question
Will anti-NFkB p105/p50/NFKB1 antibody PA2111-1 work for WB with liver?
J. Anderson
Verified customer
Asked: 2015-08-20
Answer
According to the expression profile of liver, NFKB1 is highly expressed in liver. So, it is likely that anti-NFkB p105/p50/NFKB1 antibody PA2111-1 will work for WB with liver.
Boster Scientific Support
Answered: 2015-08-20
Question
Is this PA2111-1 anti-NFkB p105/p50/NFKB1 antibody reactive to the isotypes of NFKB1?
N. Gonzalez
Verified customer
Asked: 2013-02-14
Answer
The immunogen of PA2111-1 anti-NFkB p105/p50/NFKB1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NFkB p105(193-208aa KELIRQAALQQTKEMD), different from the related rat sequence by one amino acid and from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-02-14