Product Info Summary
SKU: | M01070 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01070
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody catalog # M01070. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01070)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CEI-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human nNOS (neuronal)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01070 is reactive to NOS1 in Human, Mouse, Rat
Applications
M01070 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
160.97kDa
Background of nNOS
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of nNOS expression in Mouse brain cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded mouse kidney, using nNOS (neuronal) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For NOS1 (Source: Uniprot.org, NCBI)
Gene Name
NOS1
Full Name
Nitric oxide synthase, brain
Weight
160.97kDa
Superfamily
NOS family
Alternative Names
bNOS; brain; Constitutive NOS; EC 1.14.13.39; IHPS1; Neuronal NOS; nitric oxide synthase 1 (neuronal); nNOS; N-NOS; nNOSNOS type I; NOS; NOS1; Peptidyl-cysteine S-nitrosylase NOS1 NOS1 IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS nitric oxide synthase 1 nitric oxide synthase, brain|NOS type I|constitutive NOS|neuronal NOS|nitric oxide synthase 1 (neuronal)|peptidyl-cysteine S-nitrosylase NOS1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NOS1, check out the NOS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NOS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody (M01070)
Hello CJ!
M01070 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Distribution of constitutive nitric oxide synthase in the jejunum of adult rat
Kong L, Wei Q, Fedail Js, Shi F, Nagaoka K, Watanabe G. J Reprod Dev. 2015;61(3):219-27. Doi: 10.1262/Jrd.2014-129. Epub 2015 Mar 20. Effects Of Thyroid Hormones On The Antioxidative Status In The Uterus Of Young Adult Rats.
Zhang W, Wei Qw, Wang Zc, Ding W, Wang W, Shi Fx. J Zhejiang Univ Sci B. 2011 Jan;12(1):55-64. Doi: 10.1631/Jzus.B1000174. Cell-Specific Expression And Immunolocalization Of Nitric Oxide Synthase Isoforms And The Related Nitric Oxide/Cyclic Gmp Si...
Wu R, Yin J, Yao K, Haefliger I. Mol Vis. 2007 Apr 2;13:493-6. Inhibition By Brimonidine Of Forskolin-Induced Nitric Oxide Synthase Expression In Human Ciliary Bodies In Vitro.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-nNOS (neuronal) NOS1 Rabbit Monoclonal Antibody
Question
Our lab were satisfied with the WB result of your anti-nNOS (neuronal) Rabbit Monoclonal antibody. However we have seen positive staining in retina sarcolemma using this antibody. Is that expected? Could you tell me where is NOS1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
From what I have seen in literature, retina does express NOS1. Generally NOS1 expresses in cell membrane, sarcolemma. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048
Boster Scientific Support
Answered: 2020-01-01
Question
We ordered your anti-nNOS (neuronal) Rabbit Monoclonal antibody for IP on brain in a previous experiment. I am using mouse, and I plan to use the antibody for WB next. My lab would like examining brain as well as cerebellum in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
I looked at the website and datasheets of our anti-nNOS (neuronal) Rabbit Monoclonal antibody and I see that M01070 has been tested on mouse in both IP and WB. Thus M01070 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-23
Question
We are interested in using your anti-nNOS (neuronal) Rabbit Monoclonal antibody for arginine catabolic process studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thanks for your inquiry. This M01070 anti-nNOS (neuronal) Rabbit Monoclonal antibody is validated on mouse brain. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-nNOS (neuronal) Rabbit Monoclonal antibody M01070 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-nNOS (neuronal) Rabbit Monoclonal antibody (M01070) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
We have seen staining in rat retina. Any tips? Is anti-nNOS (neuronal) Rabbit Monoclonal antibody supposed to stain retina positively?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
According to literature retina does express NOS1. According to Uniprot.org, NOS1 is expressed in vastus lateralis, cerebellum, brain, retina, testis, skeletal muscle, among other tissues. Regarding which tissues have NOS1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7678401
Cerebellum, Pubmed ID: 7515942
Retina, Pubmed ID: 8879752
Skeletal muscle, Pubmed ID: 9791007
Testis, Pubmed ID: 9111048
Boster Scientific Support
Answered: 2019-08-16