Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody

Product Name

Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody

View all p63/TP73L Antibodies

SKU/Catalog Number

M00167

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody (Catalog # M00167). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) TP63 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00167)

Host

Rabbit

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Polyclonal

Clone Number

5D4

Isotype

IgG

Immunogen

A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00167 is reactive to TP63 in Cow, Human, Mouse, Rat

Applications

M00167 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

76.785kDa

Background of p63/TP73L

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Anti-p40 Rabbit Polyclonal Antibody.

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Formalin-fixed, paraffin-embedded Lung Squamous Cell Carcinoma stained with Anti-p40 Rabbit Polyclonal Antibody.

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Western Blot of HEK293 lysate (1) Absence and (2) Presence of immunizing peptide p40 Rabbit PAb.

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SDS-PAGE analysis purified Anti-p40 Rabbit Polyclonal Antibody.

Gene/Protein Information For TP63 (Source: Uniprot.org, NCBI)

Alternative Names

amplified in squamous cell carcinoma; Chronic ulcerative stomatitis protein; CUSP; EEC3; EEC3B(p51B); Keratinocyte transcription factor KET; KET; KETRHS; LMS; OFC8; OFC8SHFM4TP73LNBP; p40; p51; p51tumor protein p53-like; p53CP; p63; p63AIS; p73H; p73L; p73LB(p51A); SHFM4; TP53CP; TP53L; TP63; TP73L; Transformation-related protein 63; tumor protein 63; tumor protein p53-competing protein; tumor protein p63 deltaN isoform delta; tumor protein p63; Tumor protein p73-like TP63 AIS, B(p51A), B(p51B), EEC3, KET, LMS, NBP, OFC8, RHS, SHFM4, TP53CP, TP53L, TP73L, p40, p51, p53CP, p63, p73H, p73L tumor protein p63 tumor protein 63|amplified in squamous cell carcinoma|chronic ulcerative stomatitis protein|keratinocyte transcription factor KET|transformation-related protein 63|tumor protein p53-competing protein

For more info on TP63, check out the TP63 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP63: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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