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Product Info Summary
| SKU: | M00063-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00063-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal Antibody catalog # M00063-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00063-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
COH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human pan Cadherin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00063-1 is reactive to CDH1 in Human, Mouse, Rat
Applications
M00063-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
66 kDa
Calculated molecular weight
97.456kDa
Background of CDH1
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded colon cancer, using pan Cadherin Antibody .
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of pan Cadherin expression in C6 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDH1 (Source: Uniprot.org, NCBI)
Gene Name
CDH1
Full Name
Cadherin-1
Weight
97.456kDa
Alternative Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH1, check out the CDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal Antibody (M00063-1)
Hello CJ!
M00063-1 has been cited in 23 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
Species: Mouse
M00063-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND ILEUM, DILUTION:NA
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an %u03B1-smooth muscle actin-Cre %u2026
HIF%u20101%u03B1 binding to AEG%u20101 promoter induced upregulated AEG%u20101 expression associated with metastasis in ovarian cancer
Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of %u2026
Overexpression of SDF-1 activates the NF-%u03BAB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells
Placental growth factor triggers epithelial%u2010to%u2010mesenchymal transition%u2010like changes in rat type ii alveolar epithelial cells: activation of nuclear factor %u03BAB signalling %u2026
RhoC is essential in TGF-?1 induced epithelial-mesenchymal transition in cervical cancer cells.
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-pan Cadherin 1 CDH1 Rabbit Monoclonal Antibody
Question
We have been able to see staining in human placenta. Are there any suggestions? Is anti-pan Cadherin Rabbit Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
From what I have seen in literature placenta does express CDH1. From what I have seen in Uniprot.org, CDH1 is expressed in jejunal mucosa, pancreas, liver, epidermal carcinoma, hippocampus tongue, placenta, brain, among other tissues. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2020-05-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus tongue using anti-pan Cadherin Rabbit Monoclonal antibody M00063-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-12
Question
Do you have a BSA free version of anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 available?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-19
Question
Would anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 work for IF with hippocampus tongue?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
According to the expression profile of hippocampus tongue, CDH1 is highly expressed in hippocampus tongue. So, it is likely that anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 will work for IF with hippocampus tongue.
Boster Scientific Support
Answered: 2019-10-30
Question
We were well pleased with the WB result of your anti-pan Cadherin Rabbit Monoclonal antibody. However we have been able to see positive staining in jejunal mucosa cell junction using this antibody. Is that expected? Could you tell me where is CDH1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
From literature, jejunal mucosa does express CDH1. Generally CDH1 expresses in cell junction, adherens junction. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2019-09-16
Question
I was wanting to use your anti-pan Cadherin Rabbit Monoclonal antibody for IF for human hippocampus tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human hippocampus tongue identification?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody has been validated for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human hippocampus tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-13
Question
Is this M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody reactive to the isotypes of CDH1?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The immunogen of M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody is A synthesized peptide derived from human pan Cadherin . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-27
Question
I am interested in to test anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 on human hippocampus tongue for research purposes, then I may be interested in using anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-27
Answer
The products we sell, including anti-pan Cadherin Rabbit Monoclonal antibody M00063-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-27
Question
We are currently using anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
The anti-pan Cadherin Rabbit Monoclonal antibody (M00063-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-30
Question
My question regarding product M00063-1, anti-pan Cadherin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Singh
Verified customer
Asked: 2018-07-17
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-17
Question
Is a blocking peptide available for product anti-pan Cadherin Rabbit Monoclonal antibody (M00063-1)?
G. Singh
Verified customer
Asked: 2018-07-16
Answer
We do provide the blocking peptide for product anti-pan Cadherin Rabbit Monoclonal antibody (M00063-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-07-16
Question
I see that the anti-pan Cadherin Rabbit Monoclonal antibody M00063-1 works with IF, what is the protocol used to produce the result images on the product page?
J. Evans
Verified customer
Asked: 2018-06-13
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-13
Question
Our lab used your anti-pan Cadherin Rabbit Monoclonal antibody for IP on liver last year. I am using rat, and We are going to use the antibody for WB next. I am looking for examining liver as well as placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
I have checked the website and datasheets of our anti-pan Cadherin Rabbit Monoclonal antibody and it appears that M00063-1 has been tested on rat in both IP and WB. Thus M00063-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-11-15
Question
I have attached the WB image, lot number and protocol we used for hippocampus tongue using anti-pan Cadherin Rabbit Monoclonal antibody M00063-1. Please let me know if you require anything else.
G. Moore
Verified customer
Asked: 2015-04-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-07
Question
Will M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Li
Verified customer
Asked: 2013-02-08
Answer
You can see on the product datasheet, M00063-1 anti-pan Cadherin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-02-08
