Anti-Paxillin PXN Rabbit Monoclonal Antibody

Paxillin antibody

Boster Bio Anti-Paxillin PXN Rabbit Monoclonal Antibody catalog # M01033. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01033
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

Product Name

Anti-Paxillin PXN Rabbit Monoclonal Antibody

View all Paxillin Antibodies

SKU/Catalog Number

M01033

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Paxillin PXN Rabbit Monoclonal Antibody catalog # M01033. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Paxillin PXN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01033)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BIF-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Paxillin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01033 is reactive to PXN in Human, Mouse, Rat

Applications

M01033 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

64.505kDa

Background of Paxillin

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
IHC 1:50-1:100
ICC/IF 1:50-1:100
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PXN (Source: Uniprot.org, NCBI)

Gene Name

PXN

Full Name

Paxillin

Weight

64.505kDa

Superfamily

paxillin family

Alternative Names

FLJ16691; Paxillin; PXN PXN paxillin paxillin|testicular tissue protein Li 134

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PXN, check out the PXN Infographic

PXN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PXN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Find answers in Q&As, reviews.

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8 Customer Q&As for Anti-Paxillin PXN Rabbit Monoclonal Antibody

Question

Our lab used your anti-Paxillin Rabbit Monoclonal antibody for WB on fundus of stomach in a previous experiment. I am using human, and I plan to use the antibody for IP next. you antibody examining fundus of stomach as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IP?

Verified Customer

Verified customer

Asked: 2020-02-19

Answer

I have checked the website and datasheets of our anti-Paxillin Rabbit Monoclonal antibody and I see that M01033 has been validated on human in both WB and IP. Thus M01033 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-19

Question

you antibody using your anti-Paxillin Rabbit Monoclonal antibody for ras gtpase and map kinases studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

Thank you for your inquiry. This M01033 anti-Paxillin Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-22

Question

We have observed staining in mouse placenta. Are there any suggestions? Is anti-Paxillin Rabbit Monoclonal antibody supposed to stain placenta positively?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

From what I have seen in literature placenta does express PXN. From what I have seen in Uniprot.org, PXN is expressed in fundus of stomach, placenta, fetal kidney, brain testis, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039

Boster Scientific Support

Answered: 2020-01-15

Question

My colleagues were satisfied with the WB result of your anti-Paxillin Rabbit Monoclonal antibody. However we have observed positive staining in liver cytoskeleton using this antibody. Is that expected? Could you tell me where is PXN supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

From what I have seen in literature, liver does express PXN. Generally PXN expresses in cytoplasm, cytoskeleton. Regarding which tissues have PXN expression, here are a few articles citing expression in various tissues:
Brain, and Testis, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 16964243, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal kidney, Pubmed ID: 17974005
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 9054445, 14702039

Boster Scientific Support

Answered: 2019-10-01

Question

Our lab want to know about to test anti-Paxillin Rabbit Monoclonal antibody M01033 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Paxillin Rabbit Monoclonal antibody M01033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

The products we sell, including anti-Paxillin Rabbit Monoclonal antibody M01033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-13

Question

I was wanting to use your anti-Paxillin Rabbit Monoclonal antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?

N. Singh

Verified customer

Asked: 2019-04-02

Answer

As indicated on the product datasheet, M01033 anti-Paxillin Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-02

Question

Is this M01033 anti-Paxillin Rabbit Monoclonal antibody reactive to the isotypes of PXN?

A. Carter

Verified customer

Asked: 2015-07-02

Answer

The immunogen of M01033 anti-Paxillin Rabbit Monoclonal antibody is A synthesized peptide derived from human Paxillin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-07-02

Question

We are currently using anti-Paxillin Rabbit Monoclonal antibody M01033 for rat tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

C. Edwards

Verified customer

Asked: 2013-06-18

Answer

The anti-Paxillin Rabbit Monoclonal antibody (M01033) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-06-18

Order DetailsPrice
M01033

100μL

$370
M01033-30ul

30μL sample

$99
M01033-carrier-free

Carrier Free

$370

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$370.00

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