Anti-Phospho-CREB(S133) Antibody

Product Name

Anti-Phospho-CREB(S133) Antibody

View all CREB Antibodies

SKU/Catalog Number

A00577

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-CREB(S133) Antibody (Catalog # A00577). Tested in IHC-P-Leica, IF, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-Phospho-CREB(S133) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00577)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00577 is reactive to CREB1 in Human

Applications

A00577 is guaranteed for IF, WB, IHC-P-Leica Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.688kDa

Background of CREB

CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500
IHC-P-Leica: 1:500

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Click image to see more details

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Click image to see more details

Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB (S133) Antibody (upper) or tubulin (lower).

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human hepatocarcinoma tissue using A00577 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using A00577 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)

Alternative Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; pCREB; transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein

For more info on CREB1, check out the CREB1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the CREB1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis