Anti-PRDX6 Antibody Picoband™ (monoclonal, 6I8)

Product Name

Anti-PRDX6 Antibody Picoband™ (monoclonal, 6I8)

View all Peroxiredoxin 6 Antibodies

SKU/Catalog Number

M01847-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRDX6 Antibody Picoband™ (monoclonal, 6I8) catalog # M01847-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PRDX6 Antibody Picoband™ (monoclonal, 6I8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01847-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

6I8

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01847-1 is reactive to PRDX6 in Human, Mouse, Rat

Applications

M01847-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

29 kDa.

Calculated molecular weight

25.035kDa

Background of Peroxiredoxin 6

PRDX6 is also known as PRX, p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H (2)O (2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PRDX6 using anti-PRDX6 antibody (M01847-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela tissue lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human SW620 whole cell lysates.
Lane 6: rat RH35 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PRDX6 ntigen affinity purified polyclonal antibody (Catalog # M01847-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 29KD.The expected band size for PRDX6 is at 25KD.

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Figure 2. IF analysis of PRDX6 using anti-PRDX6 antibody (M01847-1).
PRDX6 was detected in immunocytochemical section of A549. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-PRDX6 Antibody (M01847-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3.Flow Cytometry analysis of Hela cells using anti-PRDX6 antibody (M01847-1).
Overlay histogram showing Hela cells stained with M01847-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PRDX6 Antibody (M01847-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Figure 4. IHC analysis of PRDX6 using anti-PRDX6 antibody (M01847-1).
PRDX6 was detected in paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PRDX6 Antibody (M01847-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For PRDX6 (Source: Uniprot.org, NCBI)

Alternative Names

1-Cys; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; aiPLA21-Cys PRX; Antioxidant protein 2; AOP2; AOP2Non-selenium glutathione peroxidase; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1.-; KIAA0106Red blood cells page spot 12; Liver 2D page spot 40; MGC46173; NSGPx; NSGPx1-Cys peroxiredoxin; p29; Peroxiredoxin 6; peroxiredoxin-6; PRDX6; PRX PRDX6 1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29 peroxiredoxin 6 peroxiredoxin-6|1-Cys PRX|1-Cys peroxiredoxin|24 kDa protein|LPC acyltransferase 5|acidic calcium-independent phospholipase A2|antioxidant protein 2|epididymis secretory sperm binding protein Li 128m|glutathione-dependent peroxiredoxin|liver 2D page spot 40|lyso-PC acyltransferase 5|lysophosphatidylcholine acyltransferase 5|non-selenium glutathione peroxidase|red blood cells page spot 12

For more info on PRDX6, check out the PRDX6 Infographic

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In this infographic, you will see the following information for PRDX6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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