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Product Info Summary
| SKU: | MA1091 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)
View all Synaptophysin Antibodies
SKU/Catalog Number
MA1091
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) catalog # MA1091. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1091)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
SVP-38
Isotype
Mouse IgG1
Immunogen
Rat retina synaptosome.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1091 is reactive to SYP in Human, Rat
Applications
MA1091 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
33.845kDa
Background of Synaptophysin
Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al. (1990) concluded that the gene has 7 exons distributed over about 20 kb. SYP mapped the SYP locus to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, rat, By Heat
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, rat, -
Western blot, 0.5-1μg/ml, Human, rat
Validation Images & Assay Conditions
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Anti-Synaptophysin antibody (monoclonal), MA1091, Western blotting
WB: Rat Brain Tissue Lysate
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Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Human meningeoma Tissue
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Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Rat Brain Tissue
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Anti-Synaptophysin antibody (monoclonal), MA1091, IHC(P)
IHC(P): Human Glioma Tissue
Protein Target Info & Infographic
Gene/Protein Information For SYP (Source: Uniprot.org, NCBI)
Gene Name
SYP
Full Name
Synaptophysin
Weight
33.845kDa
Superfamily
synaptophysin/synaptobrevin family
Alternative Names
Major synaptic vesicle protein p38; MRX; MRXSYP; Synaptophysin; SYP SYP MRX96, MRXSYP synaptophysin synaptophysin|major synaptic vesicle protein P38
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYP, check out the SYP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) (MA1091)
Hello CJ!
MA1091 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jing M,Yi Y,Jinniu Z,Xiuli K,Jianxian W.Rehabilitation training improves nerve injuries by affecting Notch1 and SYN.Open Med (Wars).2020 May 15;15(1):387-395.doi:10.1515/med-2020-0045.PMID:33335999;PMCID:PMC7712290.
Species: Rat
MA1091 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION: 1:200
Lin J,Hao C,Gong Y,Zhang Y,Li Y,Feng Z,Xu X,Huang H,Liao W. Effect of Tetramethylpyrazine on Neuroplasticity after Transient Focal Cerebral Ischemia Reperfusion in Rats. Evid Based Complement Alternat Med. 2021 Jan 18;2021:1587241.doi:10.1155/2021/1587241
Species: Rat
MA1091 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Li Xh, Xin X, Wang Y, Wu Jz, Jin Zd, Ma Ln, Nie Cj, Xiao X, Hu Y, Jin Mw. J Alzheimers Dis. 2013;34(3):755-67. Doi: 10.3233/Jad-122017. Pentamethylquercetin Protects Against Diabetes-Related Cognitive Deficits In Diabetic Goto-Kakizaki Rats.
Liu Y, Liang Z, Liu J, Zou W, Li X, Wang Y, An L. Neural Regen Res. 2013 Oct 15;8(29):2725-33. Doi: 10.3969/J.Issn.1673-5374.2013.29.004. Downregulation Of Caveolin-1 Contributes To The Synaptic Plasticity Deficit In The Hippocampus Of Aged Rats.
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Auto...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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18 Customer Q&As for Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38)
Question
Will anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 work on canine IHC with brain?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
Our lab technicians have not tested anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-05
Question
My question regards using your anti-Synaptophysin antibody (Monoclonal, SVP-38) for regulation of opioid receptor signaling pathway studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We appreciate your inquiry. This MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) is tested on rat brain tissue, tissue lysate, human meningeoma tissue, glioma tissue. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-06
Question
Our lab were happy with the WB result of your anti-Synaptophysin antibody (Monoclonal, SVP-38). However we have observed positive staining in brain secretory vesicle, using this antibody. Is that expected? Could you tell me where is SYP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
According to literature, brain does express SYP. Generally SYP expresses in cytoplasmic vesicle, secretory vesicle,. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-02-27
Question
We purchased anti-Synaptophysin antibody (Monoclonal, SVP-38) for IHC on brain in the past. I am using human, and We are going to use the antibody for WB next. We need examining brain as well as amygdala in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
I viewed the website and datasheets of our anti-Synaptophysin antibody (Monoclonal, SVP-38) and it appears that MA1091 has been tested on human in both IHC and WB. Thus MA1091 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-20
Question
Is a blocking peptide available for product anti-Synaptophysin antibody (Monoclonal, SVP-38) (MA1091)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We do provide the blocking peptide for product anti-Synaptophysin antibody (Monoclonal, SVP-38) (MA1091). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-07
Question
We are currently using anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-Synaptophysin antibody (Monoclonal, SVP-38) (MA1091) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
Do you know if MA1091 has been tested on mouse samples for WB and IHC?
Verified customer
Asked: 2019-06-19
Answer
The Anti-Synaptophysin Syp Antibody (Monoclonal, SVP-38) (MA1091) is not recommened to work on mouse samples for WB and IHC.
Boster Scientific Support
Answered: 2019-06-20
Question
See attached the WB image, lot number and protocol we used for brain using anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-07
Question
Would MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
It shows on the product datasheet, MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-06
Question
Will anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
According to the expression profile of brain, SYP is highly expressed in brain. So, it is likely that anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 will work for WB with brain.
Boster Scientific Support
Answered: 2018-12-04
Question
I was wanting to use your anti-Synaptophysin antibody (Monoclonal, SVP-38) for WB for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
O. Krishna
Verified customer
Asked: 2018-08-17
Answer
As indicated on the product datasheet, MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-17
Question
I have a question about product MA1091, anti-Synaptophysin antibody (Monoclonal, SVP-38). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-23
Question
Is this MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) reactive to the isotypes of SYP?
Verified Customer
Verified customer
Asked: 2017-11-01
Answer
The immunogen of MA1091 anti-Synaptophysin antibody (Monoclonal, SVP-38) is Rat retina synaptosome. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-01
Question
I am looking for to test anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 on human brain for research purposes, then I may be interested in using anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Banerjee
Verified customer
Asked: 2017-07-17
Answer
The products we sell, including anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-17
Question
Is there a BSA free version of anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 available?
M. Brown
Verified customer
Asked: 2017-07-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-07
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091. Let me know if you need anything else.
S. Mangal
Verified customer
Asked: 2017-03-29
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-03-29
Question
We have seen staining in human anterior cingulate cortex. Are there any suggestions? Is anti-Synaptophysin antibody (Monoclonal, SVP-38) supposed to stain anterior cingulate cortex positively?
A. Krishna
Verified customer
Asked: 2016-03-08
Answer
According to literature anterior cingulate cortex does express SYP. According to Uniprot.org, SYP is expressed in anterior cingulate cortex, amygdala, brain, among other tissues. Regarding which tissues have SYP expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-03-08
Question
I see that the anti-Synaptophysin antibody (Monoclonal, SVP-38) MA1091 works with WB, what is the protocol used to produce the result images on the product page?
T. Miller
Verified customer
Asked: 2013-11-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-11-22
