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Product Info Summary
| SKU: | A02554-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TNFSF4 Antibody
View all OX40 Ligand/TNFSF4 Antibodies
SKU/Catalog Number
A02554-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TNFSF4 Antibody (Catalog # A02554-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TNFSF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TNFSF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02554-2)
Host
Rabbit
Contents
TNFSF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
Rabbit polyclonal TNFSF4 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TNFSF4. The immunogen is located within the last 50 amino acids of TNFSF4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms.
Reactive Species
A02554-2 is reactive to TNFSF4 in Human, Mouse, Rat
Applications
A02554-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
21.05kDa
Background of OX40 Ligand/TNFSF4
The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mL
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Immunohistochemistry of TNFSF4 in human spleen tissue with TNFSF4 antibody at 5 μg/ml.
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Immunofluorescence of TNFSF4 in human spleen tissue with TNFSF4 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF4 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF4
Full Name
Tumor necrosis factor ligand superfamily member 4
Weight
21.05kDa
Superfamily
tumor necrosis factor family
Alternative Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 TNFSF4 CD134L, CD252, GP34, OX-40L, OX4OL, TNLG2B, TXGP1 TNF superfamily member 4 tumor necrosis factor ligand superfamily member 4|CD134 ligand|OX40 antigen ligand|glycoprotein Gp34|tax-transcriptionally activated glycoprotein 1 (34kD)|tumor necrosis factor (ligand) superfamily member 4|tumor necrosis factor ligand 2B|tumor necrosis factor superfamily member 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF4, check out the TNFSF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNFSF4 Antibody (A02554-2)
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