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- Table of Contents
NEDD8 Antibodies
AND NEDD8 ELISA kits, NEDD8 Proteins
Many biological assays use antibodies to detect NEDD8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NEDD8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NEDD8 antibodies...
Designing a Western blot for NEDD8? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the NEDD8 Western Blot GuideDesigning an IHC experiment for NEDD8? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the NEDD8 IHC GuideNEDD8 Infographic by Boster Bio
All NEDD8 Products
Product
Figures
Specificity
Applications
Price
Human
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00548-2
100 μg/vial
Cat # A00548-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00547
100 μg/vial
Cat # A00547
100 μg/vial
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00556-2
100 μg/vial
Cat # A00556-2
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # PB9838
100 μg/vial
Cat # PB9838
100 μg/vial
Human, Mouse, Rat
Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00553-2
100 μg/vial
Cat # A00553-2
100 μg/vial
Human
ELISA, Flow Cytometry, IF, ICC, WB
$370
Cat # A00553-3
100 μg/vial
Cat # A00553-3
100 μg/vial
Mouse, Rat
ELISA, IF, IHC, ICC, WB
$370
Cat # A00555-4
100 μg/vial
Cat # A00555-4
100 μg/vial
Human
ELISA, Flow Cytometry, IF, IHC, WB
$370
Cat # A00555-3
100 μg/vial
Cat # A00555-3
100 μg/vial
Human
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00557-2
100 μg/vial
Cat # A00557-2
100 μg/vial
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00557-1
100 μg/vial
Cat # A00557-1
100 μg/vial
Human, Mouse, Rat
ELISA
$480
Cat # EKC1396
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1396
1 kit, containing one 96-well plate and all necessary reagents
Human, Mouse
ELISA
$480
Cat # EKC1412
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1412
1 kit, containing one 96-well plate and all necessary reagents
NEDD8 Overview
Facts about NEDD8.
NEDD8
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M.
Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and consequently promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. .
Gene Information
| Human | |
|---|---|
| Gene Name: | NEDD8 |
| Uniprot: | Q15843 |
| Entrez: | 4738 |
Family
| Belongs to: |
|---|
| ubiquitin family |
Alternative Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8
Molecular Weight
Mass (kDA):
9.072 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 14q12 |
| Sequence: | 14; NC_000014.9 (24216857..24232367, complement) |
Tissue Specificity
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Subcellular Localizations
Nucleus. Mainly nuclear.
Diseases
- Neoplasms
- Malignant Neoplasms
- Cell Transformation, Neoplastic
- Carcinoma
- Von Hippel-lindau Syndrome
- Alzheimer's Disease
- Neurodegenerative Disorders
- Parkinson Disease
- Growth Arrest
- Leukemia
- Kidney Neoplasm
- Hyperplasia
Interacting Proteins
- BTRC
- CUL1
- KHNYN
- POLI
- RAD23A
- RPL11
- RPL7
- RPS20
- RPS3
- RPS7
Pathways
- Conjugation
- Cell Cycle
- Localization
- Proteolysis
- Cell Growth
- Cell Death
- Cell Proliferation
- Pathogenesis
- S Phase
- Dna Replication
- Dna Repair
- Cell Cycle Arrest
- Mitosis
PTMs
- Ubiquitination
- Cleavage
- Phosphorylation
- Sumoylation
- Acetylation
Research Areas
- Cancer
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NEDD8
bosterbio.com/bosterbio-gene-info-cards/NEDD8
References
PMID: 9694792 by Osaka F., et al. A new NEDD8-ligating system for cullin-4A.
PMID: 10318914 by Liakopoulos D., et al. Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function.
11 Publications of Boster NEDD8 Antibodies & ELISA Kits
- PubMed ID: 10.1371/journal.pone.0148536, Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated S...
- PubMed ID: 10.1097/BRS.0b013e31825d4ebc, Increased Expression of Netrin-1 and Its Deleted in Colorectal Cancer Receptor i...
- PubMed ID: 10.1039/C5AN00799B, Deformability and size-based cancer cell separation using an integrated microflu...
- PubMed ID: 10.1016/j.gendis.2019.12.012, All-trans retinoic acid reverses malignant biological behavior of hepatocarcinom...
- PubMed ID: 10.1016/j.bbrc.2017.12.096, MiR-181a contributes gefitinib resistance in non-small cell lung cancer cells by...
- PubMed ID: 10.1007/s10616-018-0188-6, Co-cultured the MSCs and cardiomyocytes can promote the growth of cardiomyocytes
- PubMed ID: 10.1007/s00795-021-00306-5, Nicorandil, an ATP-sensitive potassium channel activation, attenuates myocardial...
- PubMed ID: 10.1002/cbf.1701, Mesenchymal stem cells protect islets from hypoxia/reoxygenation‐induced injur...
- PubMed ID: 10.1002/cbf.1523, Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation
- PubMed ID: 32335297, Wang Q,Shang J,Zhang Y,Zhou Y,Tang L.MiR-451a restrains the growth and metastati...
- PubMed ID: 32456285, Jiang B,Adams Z,Moonah S,Shi H,Maupin-Furlow J,Moskovitz J.The Antioxidant Enzym...
*More publications can be found for each product on its corresponding product page