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Product Info Summary
| SKU: | EK0392 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
View all IL-1 beta/IL-1F2 ELISA kits
SKU/Catalog Number
EK0392
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0392)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: A117-S269
Sensitivity
<0.15 pg/ml
Assay Range
3.9 pg/ml - 250 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0392 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0392 is reactive to IL1B in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0392 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-1 beta/IL-1F2
Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0392-CAP | Anti-Human IL1B Pre-coated 96-well strip microplate | 1 |
| EK0392-ST | Human IL1B Standard | 2 vials, 1 ng/tube |
| EK0392-DA | Human IL1B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 3.9 | 7.8 | 15.6 | 31.2 | 62.5 | 125 | 250 |
| O.D. | 0.022 | 0.074 | 0.105 | 0.249 | 0.423 | 0.707 | 1.095 | 1.830 |
Data Example Images
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Human IL-1 beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 12.5 pg/ml..
Some articles we found to cite concentrations of IL-1 beta/IL-1F2 in samples: 22068351 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 11 | 60 | 123 | 10 | 58 | 112 |
| Standard deviation | 0.45 | 2.88 | 8.97 | 0.57 | 3.24 | 9.96 |
| CV (%) | 4.1% | 4.8% | 7.3% | 5.7% | 5.6% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 11 | 10 | 11 | 11 | 10 | 0.43 | 4.3% |
| Sample 2 | 60 | 54 | 52 | 53 | 54 | 3.11 | 5.7% |
| Sample 3 | 123 | 119 | 123 | 112 | 119 | 4.49 | 3.7% |
Protein Target Info & Infographic
Gene/Protein Information For IL1B (Source: Uniprot.Org, NCBI)
Gene Name
IL1B
Full Name
Interleukin-1 beta
Weight
30.748kDa
Superfamily
IL-1 family
Alternative Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IL1B, check out the IL1B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL1B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (EK0392)
Hello CJ!
EK0392 has been cited in 203 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury
Serum 17-OH progesterone and free testosterone levels in women patients with Familial Mediterranean Fever: a pivotal study.
Emodin attenuates adenosine triphosphate‑induced pancreatic ductal cell injury in vitro via the inhibition of the P2X7/NLRP3 signaling pathway
Pitavastatin attenuates monocyte activation in response to orthopedic implant‑derived wear particles by suppressing the NF‑κB signaling pathway
Inhibition of miR‑101‑3p protects against sepsis‑induced myocardial injury by inhibiting MAPK and NF‑κB pathway activation via the upregulation of DUSP1
Phorbol myristate acetate induces cellular senescence in rat microglia in vitro
CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells
Mechanisms of the lipopolysaccharide‑induced inflammatory response in alveolar epithelial cell/macrophage co‑culture
Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP‑1 cells
Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome
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Customer Reviews
Have you used Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®?
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2 Reviews For Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
0
User Friendly And Economical ELISA Kit--Dhruva Bhattacharya, University of Arizona, Opthalmology and Vision Science, Postdoctoral Research Associate
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | "Culture media supernatant" of transformed cultured cells of human conjunctiva epithelial cells |
| Detection | -- |
"I am very much satisfied with the product Human IL-1 Beta PicoKine™ ELISA Kit (EK0392) purchased from Boster Bio. I used the Boster Bio ElISA kit for the first time and I am very happy with this product. After trying the IL-1beta kit, I have purchased two other cytokine detection ELISA kits from Boster Bio. The methodology of Human IL-1 Beta PicoKine™ ELISA Kit is simplified and user friendly. It required minimum optimization for my experiments. The written instructions provided with the kit is very useful for running the ELISA. The results obtained so far have been consistent and reproducible. For my future ELISA experiments, I'll be buying the ELISA kit from Boster Bio."
Dhruva Bhattacharya
Verified customer
Submitted 2017-12-22
0
Good quality budget option--Milos Lazic, JECURE THERAPEUTICS, SCIENTIST II
Excellent
Source: Biocompare.com
| Applications | ELISA |
|---|---|
| Sample | protein concentrations in cell culture media |
| Detection | -- |
"We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses. This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations. Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data. I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used."
Milos Lazic
Verified customer
Submitted 2016-08-27
Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
17 Customer Q&As for Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
Question
Can below kits work with Saliva samples?
Verified customer
Asked: 2022-02-24
Answer
Our lab hasn't worked on Saliva samples using EK0392. Please, run a pilot test to see if the kits could work for the specific sample. In theory, if expression level of proteins in the samples falls in the assay range of the kit, the kits are able to detect the proteins in the samples.
Boster Scientific Support
Answered: 2022-03-28
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-14
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-1 beta in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
S. Harris
Verified customer
Asked: 2020-11-13
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 beta ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-13
Question
Does this ELISA recognize specifically the mature form of IL1-beta?
Verified customer
Asked: 2020-09-10
Answer
This ELISA does not recognize specifically the mature form of IL1-beta.
Boster Scientific Support
Answered: 2020-09-11
Question
Is there any cross-reactivity with the IL1 beta precursor pro-IL1 beta?
Verified customer
Asked: 2020-09-10
Answer
There is cross-reactivity with the IL1 beta precursor pro-IL1 beta.
Boster Scientific Support
Answered: 2020-09-11
Question
Do you offer an Alternative ELISA that is specific to the mature IL-1-beta?
Verified customer
Asked: 2020-09-10
Answer
Unfortunately, we haven't got any ELISA that is specific to the mature IL-1-beta.
Boster Scientific Support
Answered: 2020-09-11
Question
Q: if the enzyme conjugated IL-1 beta antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 beta antigen?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-18
Question
we received a customer inquiry regarding the products listed below. Our customer is interested in: ek0392 // ABIN411293 ek0410 // ABIN411303 ek0525 // ABIN411361 and ek0437 // ABIN411318 Do you know if your Kit has been tested for colostrum/breast milk or might work with breast milk samples as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We haven't tested ek0392, ek0410 ek0525 , and ek0437 on colostrum/breast milk. We are uncertain if the kits are able to work on colostrum/breast milk. Please suggest the custome run pilot test if he wants to try. In theory, if the expression level of the targets protein in the colostrum/breast milk falls in the assay range of the kits, the kits are able to detect the protein in the sample.
Boster Scientific Support
Answered: 2019-12-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
D. Babu
Verified customer
Asked: 2019-09-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-09-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
T. Williams
Verified customer
Asked: 2019-05-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-05-05
Question
Have you tested EK0392 in tissue homogenates and Cell culture supernates?
Verified customer
Asked: 2019-03-15
Answer
The Human IL-1 beta EK0392 Elisa Kit has not been tested on tissue homogenates. Endogenous biotin in tissue homogenates might introduce a false positive signal. We recommend to run a pilot test to confirm the effect of the endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-03-18
Question
Q: What is the optimal O.D. value for IL-1 beta ELISA kit? I used your IL-1 beta ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-1 beta even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-1 beta ELISA kit to have sensitivity of 0.15pg/ml, that means the minimum amount of IL-1 beta that can be declared/interpreted as positive by the above standard is 0.15pg/ml.
Boster Scientific Support
Answered: 2018-09-17
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
V. Scott
Verified customer
Asked: 2018-05-21
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-05-21
Question
For EK0392, what is the suggested dilution for plasma samples? Also, has this kit been tested for brain tissues or tissues in general. Do you recommend the customer to use brain tissue samples with this kit? suggestions?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
The suggested dilution for plasma samples is 1:1. EK0392 hasn't been tested for any tissues. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2017-11-09
Question
Hi, i want to use your ELISA kits in my study. I will use human serum in depression patients. And i want to measure IL-1beta(EK0392), BDNF(EK0307), TNF-alpha(EK0525) and Interferon-Gamma(EK0373) with BOSTER kits. Do you recommend any dilutions for my samples with these ELISA kits of yours? Or should i make my measures directly, without any dilution?
Verified Customer
Verified customer
Asked: 2015-11-10
Answer
Our lab technicans haven't woked on
Boster Scientific Support
Answered: 2015-11-10
Question
Hello Support: I need to know how I can prepare the product EK0392 diluted into 80 fold in reagent preparation that is enough for 96 well, I need the volume. Thanks!
Verified Customer
Verified customer
Asked: 2014-04-02
Answer
For the product EK0392 you will need a 100ul of antibody for each 96 well plate. So if you are going to use all the 96 well plate, you need 9600ul (9.6ml) volume of antibody. Usually there is a loss of few micro liters while pipetting, so we suggest preparing more than what it is required.
Boster Scientific Support
Answered: 2014-04-02
Question
Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?
K. Carter
Verified customer
Asked: 2014-03-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2014-03-11
