Product Info Summary
| SKU: | PROTP06804 |
|---|---|
| Size: | Starting from 10ug |
| Source: | Baculovirus |
| Application: | SDS-PAGE, Bioactivity |
Product info
Product Name
Mouse TNF-alpha Recombinant Protein
View all Tnf recombinant proteins
SKU/Catalog Number
PROTP06804
Size
Starting from 10ug
Tag
His-Tag
Description
Mouse TNF-alpha Recombinant Protein expressed in Baculovirus with His-tag. Sequence domain: 80-235aa. Application(s): SDS-PAGE, Bioactivity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)
Cite This Product
Mouse TNF-alpha Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP06804)
Form
Liquid
Formulation
Phosphate-Buffered Saline (pH 7.4)
Concentration
1mg/ml (determined by absorbance at 280nm)
Purity
> 90% by SDS-PAGE
Predicted MW
25.9 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Mouse, P06804, 80-235aa; LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HHBioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range is less or equal to 0.02 ng/ml.Assay dilution & Images
Validation Images & Assay Conditions
Click image to see more details
3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
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