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Product Info Summary
| SKU: | A01263 |
|---|---|
| Size: | 200ug |
| Reactive Species: | Human, Mouse, Leopard frog |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-beta Actin ACTB Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
A01263
Size
200ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-beta Actin ACTB Antibody (Catalog # A01263). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Leopard Frog.
Storage & Handling
Store beta-Actin Loading Control Antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-beta Actin ACTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01263 is reactive to ACTB in Human, Mouse, Leopard frog
Applications
A01263 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.737kDa
Background of beta-Actin
In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:40,000
IF Microscopy: 1:500 - 1:2,000
WB: 1:1,000 - 1:4,000
Validation Images & Assay Conditions
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Western blot analysis of beta Actin expression in MW marker (lane 1), human embryonic kidney 293 cell lysates (lane 2), human lung carcinoma A549 cell lysates (lane 3) and mouse brain extract (lane 4). Load: 35
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Beta Actin was detected in paraffin-embedded sections of rana pipiens tissues using rabbit anti-beta Actin affinity purified polyclonal antibody (Catalog # A01263) at 1:200. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Actin ACTB Antibody (A01263)
Hello CJ!
A01263 has been cited in 192 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Activation of liver X receptor promotes hippocampal neurogenesis and improves long‐term cognitive function recovery in acute cerebral ischemia‐reperfusion mice
Gao H,Wang D,Wang YL,Mao JP,Jiang S,Yang XL.Pramipexole attenuates 6-OHDA-induced Parkinson‘s disease by mediating the Nurr1/NF-κB pathway.Mol Biol Rep.2021 Apr 23.doi:10.1007/s11033-021-06343-8.Epub ahead of print.PMID:33891271.
Species: Rat
A01263 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Peng L,Liang Y,Zhong X,Liang Z,Tian Y,Li S,Liang J,Wang R,Zhong Y,Shi Y,Zhang X.Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma.Int J Nanomedicine.2020 Feb 28;15:1363-1372.doi:
Species: Human,Mouse
A01263 usage in article: APP:WB, SAMPLE:U87-EGFRVIII CELL, DILUTION:NA
Jianming Lu,Chuanfan Zhong,Fangpeng Shu et al.HnRNP-L regulated circCSPP1/miR-520h/EGR1 axis modulates autophagy and promotes progression in prostate cancer, 19 January 2021, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3
Species: Human,Mouse
A01263 usage in article: APP:WB, SAMPLE:CANCER CELLS, DILUTION:NA
Liu L,Zhang J,Liu Y. MicroRNA-1323 serves as a biomarker in gestational diabetes mellitus and aggravates high glucose-induced inhibition of trophoblast cell viability by suppressing TP53INP1. Exp Ther Med. 2021 Mar;21(3):230. doi:10.3892/etm.2021.9661.Epu
Species: Human
A01263 usage in article: APP:WB, SAMPLE:HTR‑8/SVNEO CELL AND BEWO CELL, DILUTION:1:500
Li,J.,& Li,A. (2020).Role of microRNA 4717, its effects on programmed cell death protein-1 in hepatitis B infection, and interaction between PDCD1 and miR-4717.European Journal of Inflammation.https://doi.org/10.1177/2058739220934604
Species: Human
A01263 usage in article: APP:WB, SAMPLE:HEPG2 CELL AND LIVER TISSUE, DILUTION:1:1000
Chen,S.,Li, K.,Jiang, J. et al. Low expression of organic anion-transporting polypeptide 1B3 predicts a poor prognosis in hepatocellular carcinoma. World J Surg Onc 18, 127(2020). https://doi.org/10.1186/s12957-020-01891-y
Species: Human
A01263 usage in article: APP:WB, SAMPLE:HCC TISSUE, DILUTION:1:1000
Lu C,Fu L,Qian X,Dou L,Cang S.Knockdown of circular RNA circ-FARSA restricts colorectal cancer cell growth through regulation of miR-330-5p/LASP1 axis.Arch Biochem Biophys.2020 Aug 15;689:108434.doi:10.1016/j.abb.2020.108434.Epub 2020 May 29.PMID:32473899
Species: Human,Mouse
A01263 usage in article: APP:WB, SAMPLE:HCT116 CELL AND SW480 CELL, DILUTION:NA
Yu Y,Wang Y,Fei X,Song Z,Xie F,Yang F,Liu X,Xu Z,Wang G.All-Trans Retinoic Acid Prevented Vein Grafts Stenosis by Inhibiting Rb-E2F Mediated Cell Cycle Progression and KLF5-RARα Interaction in Human Vein Smooth Muscle Cells.Cardiovasc Drugs Ther.2020 Oct
Species: Human,New Zealand White Rabbit
A01263 usage in article: APP:WB, SAMPLE:HUVMSCS, DILUTION:1:1000
Yuan Y,Li B,Kuang Y,Ni S,Zhuge A,Yang J,Lv L,Gu S,Yan R,Li Y,Wang K,Yang L,Zhu X,Wu J,Bian X,Li L. The fiber metabolite butyrate reduces gp130 by targeting TRAF5 in colorectal cancer cells. Cancer Cell Int.2020 Jun 3;20:212.doi:10. 1186/s12935-020-01305-9
Species: Human,Mouse
A01263 usage in article: APP:WB, SAMPLE:HCT-116 CELL AND HT-29 CELL, DILUTION:1:1000
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