Product Info Summary
SKU: | H01263 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated)
View all beta-Actin Antibodies
SKU/Catalog Number
H01263
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) catalog # H01263. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01263)
Host
Mouse
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17A12
Isotype
IgG2b
Immunogen
A synthesized peptide derived from human beta Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
H01263 is reactive to ACTB in Human, Mouse, Rat
Applications
H01263 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
41.737kDa
Background of beta-Actin
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:3000-1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of beta Actin expression in (1) Hela cell lysate, (2) Human liver lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) PC12 cell lysate, (6) Rat heart lysate.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Actin Mouse Monoclonal Antibody(HRP Conjugated) (H01263)
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No publications found for H01263
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