Anti-CD252/Tnfsf4 Antibody Picoband™

Product Name

Anti-CD252/Tnfsf4 Antibody Picoband™

View all OX40 Ligand/TNFSF4 Antibodies

SKU/Catalog Number

A02554-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD252/Tnfsf4 Antibody Picoband™ catalog # A02554-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD252/Tnfsf4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02554-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse CD252 recombinant protein (Position: Q49-L198). Mouse CD252 shares 42.7% and 78.8% amino acid (aa) sequence identity with human and rat CD252, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02554-1 is reactive to TNFSF4 in Mouse, Rat

Applications

A02554-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

21-25 kDa

Calculated molecular weight

21.05kDa

Background of OX40 Ligand/TNFSF4

OX40L (TNFSF4) is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CD252 using anti-CD252 antibody (A02554-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse CD252 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD252 antigen affinity purified polyclonal antibody (Catalog # A02554-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD252 at approximately 21-25KD. The expected band size for CD252 is at 18KD.

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Figure 2. IHC analysis of CD252 using anti-CD252 antibody (A02554-1).
CD252 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CD252 Antibody (A02554-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For TNFSF4 (Source: Uniprot.org, NCBI)

Alternative Names

CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 TNFSF4 CD134L, CD252, GP34, OX-40L, OX4OL, TNLG2B, TXGP1 TNF superfamily member 4 tumor necrosis factor ligand superfamily member 4|CD134 ligand|OX40 antigen ligand|glycoprotein Gp34|tax-transcriptionally activated glycoprotein 1 (34kD)|tumor necrosis factor (ligand) superfamily member 4|tumor necrosis factor ligand 2B|tumor necrosis factor superfamily member 4

For more info on TNFSF4, check out the TNFSF4 Infographic

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In this infographic, you will see the following information for TNFSF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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