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Product Info Summary
| SKU: | M00184-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315
View all NCAM-1/CD56 Antibodies
SKU/Catalog Number
M00184-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315 (Catalog # M00184-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00184-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM315
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD56.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human CD56 (NCAM1). It may also react to mouse CD56, as predicted by immunogen homology.
Reactive Species
M00184-2 is reactive to NCAM1 in Human
Applications
M00184-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
94.574kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of human brain tissue lysate using anti-CD56 rabbit monoclonal antibody (Clone RM315) at a 1:2000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue section using anti-CD56 rabbit monoclonal antibody (Clone RM315) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For NCAM1 (Source: Uniprot.org, NCBI)
Gene Name
NCAM1
Full Name
Neural cell adhesion molecule 1
Weight
94.574kDa
Alternative Names
CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM NCAM1 CD56, MSK39, NCAM neural cell adhesion molecule 1 neural cell adhesion molecule 1|antigen recognized by monoclonal antibody 5.1H11|neural cell adhesion molecule, NCAM
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCAM1, check out the NCAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315 (M00184-2)
Hello CJ!
No publications found for M00184-2
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Customer Reviews
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Customer Q&As
Have a question?
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16 Customer Q&As for Anti-CD56 NCAM1 Rabbit Monoclonal Antibody, Clone#RM315
Question
See below the WB image, lot number and protocol we used for neocortex using anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2. Please let me know if you require anything else.
C. Collins
Verified customer
Asked: 2020-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
From what I have seen in literature liver does express NCAM1. From what I have seen in Uniprot.org, NCAM1 is expressed in neocortex, skeletal muscle, testis, brain, brain, kidney ovary, plasma, liver, among other tissues. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2020-02-26
Question
Will anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 work for WB with neocortex?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
According to the expression profile of neocortex, NCAM1 is highly expressed in neocortex. So, it is likely that anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 will work for WB with neocortex.
Boster Scientific Support
Answered: 2019-11-12
Question
We are currently using anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
C. Anderson
Verified customer
Asked: 2019-10-14
Answer
The anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 (M00184-2) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-14
Question
I am looking for using your anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 for interferon gamma signaling studies. Has this antibody been tested with western blotting on human brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We appreciate your inquiry. This M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 is validated on human brain tissue, tissue lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-09
Question
I see that the anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-26
Question
We have tried in the past anti-CD56 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
I looked at the website and datasheets of our anti-CD56 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-08-23
Question
My question regarding product M00184-2, anti-CD56 Rabbit Monoclonal antibody, Clone#RM315. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-22
Question
I was wanting to use your anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 for WB for human neocortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human neocortex identification?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
You can see on the product datasheet, M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human neocortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
My boss were content with the WB result of your anti-CD56 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
According to literature, liver does express NCAM1. Generally NCAM1 expresses in isoform 1: cell membrane. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057
Boster Scientific Support
Answered: 2019-07-16
Question
We need to test anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 on human neocortex for research purposes, then I may be interested in using anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The products we sell, including anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-10
Question
Would M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Kulkarni
Verified customer
Asked: 2019-01-09
Answer
It shows on the product datasheet, M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neocortex using anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-25
Question
Is this M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 reactive to the isotypes of NCAM1?
P. Carter
Verified customer
Asked: 2017-06-20
Answer
The immunogen of M00184-2 anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 is A peptide corresponding to the C-terminus of human CD56. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-20
Question
Do you have a BSA free version of anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 available?
B. Li
Verified customer
Asked: 2014-08-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 M00184-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-08-05
Question
Is a blocking peptide available for product anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 (M00184-2)?
A. Patel
Verified customer
Asked: 2013-08-01
Answer
We do provide the blocking peptide for product anti-CD56 Rabbit Monoclonal antibody, Clone#RM315 (M00184-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-08-01
