Anti-Human Ki67 Antibody

Product Name

Anti-Human Ki67 Antibody

View all Ki67/MKI67 Antibodies

SKU/Catalog Number

M00254-3

Size

100ul

Description

Boster Bio Anti-Human Ki67 Antibody catalog # M00254-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human Ki67 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00254-3)

Host

Rabbit

Contents

Serum with 5mM NaN₃

Clonality

Polyclonal

Immunogen

Recombinant human construct containing amino acids 1,111-1,490 expressed in and purified from E. coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00254-3 is reactive to MKI67 in Human, Mouse, Rat

Applications

M00254-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

358.694kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-5,000. IF 1:2,000-5,000, IHC 1:1,000

Validation Images & Assay Conditions

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Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki-67 M00254-3, dilution 1:10,000, (green): [1] protein standard (red), [2] HeLa cells, [3] HEK293 cells. Strong double bands above 250kDa correspond to the two major Ki-67 isoforms of molecular weight 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also detected on the blot.

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Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki-67 M00254-3, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. The Ki-67 protein accumulates

Gene/Protein Information For MKI67 (Source: Uniprot.org, NCBI)

Alternative Names

antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; antiKi67; Ki67 ihc; Ki-67 ihc; Ki67 mouse; Ki-67 mouse; Ki67 western blot; Ki-67 western blot; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126 MKI67 KIA, MIB-, MIB-1, PPP1R105 marker of proliferation Ki-67 proliferation marker protein Ki-67|antigen Ki67|antigen identified by monoclonal antibody Ki-67|proliferation-related Ki-67 antigen|protein phosphatase 1, regulatory subunit 105

For more info on MKI67, check out the MKI67 Infographic

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In this infographic, you will see the following information for MKI67: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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