Product Info Summary
| SKU: | A00139 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-9 MMP9 Antibody
SKU/Catalog Number
A00139
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody (Catalog # A00139). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Matrix metalloproteinase-9 MMP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00139)
Host
Rabbit
Contents
MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9. The immunogen is located within amino acids 260 - 310 of MMP9 .
Cross-reactivity
MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
Reactive Species
A00139 is reactive to MMP9 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
78.5 kDa
Background of MMP9
The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00139 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 μg/ml.
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Immunohistochemistry of MMP9 in human lung tissue with MMP9 antibody at 2.5 μg/mL.
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Immunofluorescence of MMP9 in human lung tissue with MMP9 antibody at 20 μg/mL.
Specific Publications For Anti-Matrix metalloproteinase-9 MMP9 Antibody (A00139)
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