Click image to see more details
Product Info Summary
| SKU: | RP1021 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Prolactin Prl Antibody
SKU/Catalog Number
RP1021
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prolactin Prl Antibody catalog # RP1021. Tested in ELISA, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prolactin Prl Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1021)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Prolactin recombinant protein (Position: L30-C226).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1021 is reactive to PRL in Mouse
Applications
RP1021 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
12 kDa
Calculated molecular weight
25.876kDa
Background of Prolactin
Prolactin (PRL) also known as luteotropic hormone (LTH) is a protein that in humans is encoded by the PRL gene. Prolactin is a peptide hormone discovered by Henry Friesen. Although it is perhaps best known for its role in lactation, prolactin already existed in the oldest known vertebrates-fishes-where its most important functions were probably related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Prolactin expression in mouse testis extract (lane 1). Prolactin at 26KD was detected using rabbit anti-Prolactin Antigen Affinity purified polyclonal antibody (Catalog # RP1021) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For PRL (Source: Uniprot.org, NCBI)
Gene Name
PRL
Full Name
Prolactin
Weight
25.876kDa
Superfamily
somatotropin/prolactin family
Alternative Names
PRL; Prolactin PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRL, check out the PRL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prolactin Prl Antibody (RP1021)
Hello CJ!
RP1021 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xianglong Meng,Chenzi Lyu,Junnan Ma,Xiaoyan Zhang,Cong Hu,Xiaojuan Su,Chenxu Ning,Wenbin Xie,Shuosheng Zhang,"Metabolomics and Network Pharmacology-Based Investigation into the Mechanisms Underlying the Therapeutic Effect of a New Chinese Traditional Medicine (Cui Nai Ling) on Bromocriptine-Induced Hypogalactia",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 8857449,17 pages,2021.https://doi.org/10.1155/2021/8857449
Species: Rat
RP1021 usage in article: APP:WB, SAMPLE:MAMMARY GLAND TISSUE, DILUTION:NA
Positive Regulation of Decidualization by l-Type Amino Acid Transporter 1 (lat1) in Pregnant Mice
MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1? induced in human endometriosis stromal cells in vitro
Huang F, Wang H, Zou Y, Liu Q, Cao J, Yin T. Int J Clin Exp Pathol. 2013 Oct 15;6(11):2487-96. Ecollection 2013. Effect Of Gnrh-Ii On The Esc Proliferation, Apoptosis And Vegf Secretion In Patients With Endometriosis In Vitro.
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Customer Reviews
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1 Reviews For Anti-Prolactin Prl Antibody
0
Specific and low variability between lots--Tabitha Doci, ELISA DEPARTMENT, MOLECULAR INNOVATIONS, ELISA SPECIALIST
Excellent
Source: Biocompare.com
| Applications | ELISA |
|---|---|
| Sample | Purified mouse prolactin and plasma |
| Detection | TMB Substrate |
"We were looking for an antibody that detects mouse prolactin in ELISA, this antibody performs well and I particularly liked that there's no lot to lot variability. Low background and fast high OD. Will purchase again."
Tabitha Doci
Verified customer
Submitted 2016-08-24
Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Anti-Prolactin Prl Antibody
Question
Is this RP1021 anti-Prolactin antibody reactive to the isotypes of PRL?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The immunogen of RP1021 anti-Prolactin antibody is E. coli-derived mouse Prolactin recombinant protein(Position: L30-C226). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-Prolactin antibody RP1021. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-25
Question
Does anti-Prolactin antibody RP1021 work on feline ELISA with mammary gland?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
Our lab technicians have not validated anti-Prolactin antibody RP1021 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Prolactin antibody RP1021 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline mammary gland in ELISA, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Does RP1021 cross-react with rat?
Verified customer
Asked: 2019-10-14
Answer
Unfortunately, our lab hasn't tested the Anti-Prolactin Prl Antibody (RP1021) cross reactivity with rat. Please see the sequence homology with rat. https://www.uniprot.org/blast/uniprot/B20191016216DA2B77BFBD2E6699CA9B6D1C41EB20C2AD9C
Boster Scientific Support
Answered: 2019-10-15
Question
Would anti-Prolactin antibody RP1021 work for WB with testis?
B. Evans
Verified customer
Asked: 2019-06-06
Answer
According to the expression profile of testis, PRL is highly expressed in testis. So, it is likely that anti-Prolactin antibody RP1021 will work for WB with testis.
Boster Scientific Support
Answered: 2019-06-06
Question
Is a blocking peptide available for product anti-Prolactin antibody (RP1021)?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do provide the blocking peptide for product anti-Prolactin antibody (RP1021). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-Prolactin antibody RP1021 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
The anti-Prolactin antibody (RP1021) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-31
Question
I see that the anti-Prolactin antibody RP1021 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-25
