Anti-NFkB-p105 (Ab-927) NFKB1 Antibody

Product Name

Anti-NFkB-p105 (Ab-927) NFKB1 Antibody

View all NFKB1 Antibodies

SKU/Catalog Number

A00283-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (Catalog # A00283-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NFkB-p105 (Ab-927) NFKB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00283-1)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Peptide sequence around aa.925~929 (C-D-S-G-V ) derived from Human NFkB-p105.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00283-1 is reactive to NFKB1 in Human, Mouse, Rat

Applications

A00283-1 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

105.356kDa

Background of NFKB1

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Validation Images & Assay Conditions

Click image to see more details

Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p105(Ab-927) Antibody #A00283-1.

Click image to see more details

Figure 2. Western blot analysis of NFKB1 using anti-NFKB1 antibody (A00283-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKB1 antigen affinity purified polyclonal antibody (Catalog # A00283-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for NFKB1.

Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B; NF-kappabeta; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151; p105; p50 NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

For more info on NFKB1, check out the NFKB1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the NFKB1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis