Anti-Perforin Prf1 Antibody (Monoclonal, CE2.10)

Product Name

Anti-Perforin Prf1 Antibody (Monoclonal, CE2.10)

View all Perforin Antibodies

SKU/Catalog Number

M01429

Size

100μg/vial

Form

Liquid

Description

Boster Bio Anti-Perforin Prf1 Antibody (Monoclonal, CE2.10) (Catalog# M01429). Tested in IP, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Perforin Prf1 Antibody (Monoclonal, CE2.10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01429)

Host

Mouse

Contents

Liquid. In PBS containing 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

CE2.10

Isotype

IgG2a

Immunogen

Recombinant mouse perforin (aa 98-534)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~85kDa.

Reactive Species

M01429 is reactive to PRF1 in Human, Mouse

Applications

M01429 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

61.377kDa

Background of Perforin

PRF1, also known as Perforin-1, is a protein that in humans is encoded by the PRF1 gene. It is mapped to 10q22.1. PRF1 is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, PRF1 inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. PRF1 has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. PRF1 is thought to act by creating holes in the plasma membrane which triggers an influx of calcium and initiates membrane repair mechanisms. These repair mechanisms bring perforin and granzymes into early endosomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunoprecipitation: human and mouse
Western Blot: mouse only
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Schematic structure of mouse perforin (recombinant, aa 98-534). The antibody recognizes an epitope within aa 402-534.

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Western blot analysis of Perforin expression in control HEK 293 whole cell lysates (lane 1), mouse T cell clone B6.1 lysates (lane 2). Perforin migrates around 66kDa was detected using mouse anti-Perforin monoclonal antibody (Catalog # M01429) at 1

Gene/Protein Information For PRF1 (Source: Uniprot.org, NCBI)

Alternative Names

Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1 PRF1 HPLH2, P1, PFP perforin 1 perforin-1|cytolysin|lymphocyte pore-forming protein|perforin 1 (pore forming protein)

For more info on PRF1, check out the PRF1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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