Anti-Phospho-p62 (Ser28) SQSTM1 Antibody

Product Name

Anti-Phospho-p62 (Ser28) SQSTM1 Antibody

View all p62/SQSTM1 Antibodies

SKU/Catalog Number

P00300-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Catalog # P00300-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-p62 (Ser28) SQSTM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00300-1)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser28 of human p62, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00300-1 is reactive to SQSTM1 in Human

Applications

P00300-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

47.687kDa

Background of p62/SQSTM1

p62, also known as sequestosome1 (SQSTM1), is a shuttle protein transporting polyubiquitinated proteins for both proteasomal and lysosomal degradation. p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease (Nogalaska et al., 2009). p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages (Nakaso et al., 2004). Phosphorylation of Ser-28 has recently been demonstrated to be related to the pathogenesis of Parkinson’s disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of Jurkat cell lysate showing specific immunolabeling of the ~48 kDa p62 phosphorylated at Ser²⁸ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Gene/Protein Information For SQSTM1 (Source: Uniprot.org, NCBI)

Alternative Names

A170; Autophagy Receptor P62; DMRV; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; EBIAP; FTDALS3; NADGP; ORCA; OSIL; oxidative stress induced like; p60PDB3; p62; p62B; Paget disease of bone 3; PDB3; phosphotyrosine independent ligand for the Lck SH2 domain p62; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Sequestosome 1; sequestosome-1; SQSTM1; Ubiquitin-binding protein p62; ZIP3 SQSTM1 A170, DMRV, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B sequestosome 1 sequestosome-1|EBI3-associated protein of 60 kDa|EBI3-associated protein p60|EBIAP|autophagy receptor p62|oxidative stress induced like|phosphotyrosine independent ligand for the Lck SH2 domain p62|phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa|ubiquitin-binding protein p62

For more info on SQSTM1, check out the SQSTM1 Infographic

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In this infographic, you will see the following information for SQSTM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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