Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody

Boster Bio Anti-S6 Ribosomal Protein (Ab-235) RPS6 Antibody catalog # A01567. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A01567
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

Product Name

Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody

See all Ribosomal Protein S6/RPS6 products

SKU/Catalog Number

A01567

Size

100μl

Form

Liquid

Description

Boster Bio Anti-S6 Ribosomal Protein (Ab-235) RPS6 Antibody catalog # A01567. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01567)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01567 is reactive to RPS6 in Human, Mouse, Rat

Applications

A01567 is guaranteed for IF, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:1000
IHC, 1:50-1:100
Immunofluorescence, 1:100-1:200

Validation Images & Assay Conditions

Gene/Protein Information For RPS6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P62753

Gene ID

6194

Gene Name

RPS6

Full Name

40S ribosomal protein S6

Weight

28.681kDa

Superfamily

eukaryotic ribosomal protein eS6 family

Alternative Names

Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6 RPS6 S6 ribosomal protein S6 40S ribosomal protein S6|phosphoprotein NP33|small ribosomal subunit protein eS6

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on RPS6, check out the RPS6 Infographic

RPS6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for RPS6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A01567

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15 Customer Q&As for Anti-S6 Ribosomal Protein(Ab-235) RPS6 Antibody

Question

Is this A01567 anti-S6 Ribosomal Protein(Ab-235) antibody reactive to the isotypes of RPS6?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

The immunogen of A01567 anti-S6 Ribosomal Protein(Ab-235) antibody is Peptide sequence around aa. 233~237 (R-L-S-S-L) derived from Human S6 Ribosomal Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-10

Question

Does anti-S6 Ribosomal Protein(Ab-235) antibody A01567 work for IHC with colon adenocarcinoma?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

According to the expression profile of colon adenocarcinoma, RPS6 is highly expressed in colon adenocarcinoma. So, it is likely that anti-S6 Ribosomal Protein(Ab-235) antibody A01567 will work for IHC with colon adenocarcinoma.

Boster Scientific Support

Answered: 2020-03-27

Question

I have a question about product A01567, anti-S6 Ribosomal Protein(Ab-235) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01567 anti-S6 Ribosomal Protein(Ab-235) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-26

Question

Do you have a BSA free version of anti-S6 Ribosomal Protein(Ab-235) antibody A01567 available?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S6 Ribosomal Protein(Ab-235) antibody A01567 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-11

Question

Is a blocking peptide available for product anti-S6 Ribosomal Protein(Ab-235) antibody (A01567)?

Verified Customer

Verified customer

Asked: 2020-03-10

Answer

We do provide the blocking peptide for product anti-S6 Ribosomal Protein(Ab-235) antibody (A01567). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-10

Question

We are interested in to test anti-S6 Ribosomal Protein(Ab-235) antibody A01567 on human colon adenocarcinoma for research purposes, then I may be interested in using anti-S6 Ribosomal Protein(Ab-235) antibody A01567 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-30

Answer

The products we sell, including anti-S6 Ribosomal Protein(Ab-235) antibody A01567, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-30

Question

I was wanting to use your anti-S6 Ribosomal Protein(Ab-235) antibody for IHC for human colon adenocarcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon adenocarcinoma identification?

Verified Customer

Verified customer

Asked: 2020-01-23

Answer

It shows on the product datasheet, A01567 anti-S6 Ribosomal Protein(Ab-235) antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon adenocarcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon adenocarcinoma using anti-S6 Ribosomal Protein(Ab-235) antibody A01567. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-26

Question

I was wanting to use using your anti-S6 Ribosomal Protein(Ab-235) antibody for ribosomal scanning and start codon recognition studies. Has this antibody been tested with western blotting on lung carcinoma tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

I appreciate your inquiry. This A01567 anti-S6 Ribosomal Protein(Ab-235) antibody is tested on lung carcinoma tissue, 293 cells, mcf7 cells. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-12

Question

See attached the WB image, lot number and protocol we used for colon adenocarcinoma using anti-S6 Ribosomal Protein(Ab-235) antibody A01567. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-31

Question

We have seen staining in mouse connective tissue. What should we do? Is anti-S6 Ribosomal Protein(Ab-235) antibody supposed to stain connective tissue positively?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

Based on literature connective tissue does express RPS6. Based on Uniprot.org, RPS6 is expressed in connective tissue, colon adenocarcinoma, colon, muscle, pancreas, skin testis, placenta, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have RPS6 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon, Muscle, Pancreas, Skin, and Testis, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8706699

Boster Scientific Support

Answered: 2019-06-25

Question

Would A01567 anti-S6 Ribosomal Protein(Ab-235) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

As indicated on the product datasheet, A01567 anti-S6 Ribosomal Protein(Ab-235) antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-05

Question

I see that the anti-S6 Ribosomal Protein(Ab-235) antibody A01567 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-09-13

Question

Our lab used your anti-S6 Ribosomal Protein(Ab-235) antibody for IHC on connective tissue a few years ago. I am using mouse, and We want to use the antibody for WB next. you antibody examining connective tissue as well as colon in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

S. Kulkarni

Verified customer

Asked: 2016-05-20

Answer

I have checked the website and datasheets of our anti-S6 Ribosomal Protein(Ab-235) antibody and I see that A01567 has been tested on mouse in both IHC and WB. Thus A01567 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2016-05-20

Question

We are currently using anti-S6 Ribosomal Protein(Ab-235) antibody A01567 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

O. Evans

Verified customer

Asked: 2013-07-08

Answer

The anti-S6 Ribosomal Protein(Ab-235) antibody (A01567) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-07-08

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A01567
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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