Anti-SMURF2 Antibody

Product Name

Anti-SMURF2 Antibody

View all SMURF2 Antibodies

SKU/Catalog Number

A02585

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SMURF2 Antibody (Catalog # A02585). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SMURF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-SMURF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02585)

Host

Rabbit

Contents

SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

SMURF2 antibody was raised against a 17 amino acid peptide near the amino terminus of human SMURF2. The immunogen is located within amino acids 140 - 190 of SMURF2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Reactive Species

A02585 is reactive to SMURF2 in Human, Mouse, Rat

Applications

A02585 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

86.196kDa

Background of SMURF2

SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of SMURF2 in C2C12 cell lysate with SMURF2 antibody at 1 μg/ml.

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Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 5 μg/mL.

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Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/mL.

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Immunofluorescence of SMURF2 in human uterus tissue with SMURF2 antibody at 20 μg/ml.

Green: SMURF2 Antibody (A02585)
Blue: DAPI staining

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Immunofluorescence of SMURF2 in mouse liver tissue with SMURF2 antibody at 20 μg/ml.

Green: SMURF2 Antibody (A02585)
Blue: DAPI staining

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Immunohistochemistry of SMURF2 in human uterus tissue with SMURF2 antibody at 2 μg/ml.

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Immunohistochemistry of SMURF2 in mouse liver tissue with SMURF2 antibody at 2 μg/ml.

Gene/Protein Information For SMURF2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686F0270; E3 ubiquitin ligase SMURF2; E3 ubiquitin-protein ligase SMURF2; EC 6.3.2; EC 6.3.2.-; hSMURF2; MGC138150; SMAD specific E3 ubiquitin protein ligase 2; SMAD ubiquitination regulatory factor 2; SMAD-specific E3 ubiquitin-protein ligase 2; SMURF2 SMURF2 SMAD specific E3 ubiquitin protein ligase 2 E3 ubiquitin-protein ligase SMURF2|E3 ubiquitin ligase SMURF2|HECT-type E3 ubiquitin transferase SMURF2|SMAD ubiquitination regulatory factor 2|hSMURF2

For more info on SMURF2, check out the SMURF2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SMURF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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