Anti-SOD2 Antibody Picoband™

Product Name

Anti-SOD2 Antibody Picoband™

View all SOD2/Mn-SOD Antibodies

SKU/Catalog Number

PB9442

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SOD2 Antibody Picoband™ catalog # PB9442. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SOD2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9442)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SOD2, different from the related mouse sequence by one amino acid, and from the related rat sequence by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9442 is reactive to SOD2 in Human, Mouse, Rat

Applications

PB9442 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

24 kDa

Calculated molecular weight

24.75kDa

Background of SOD2/Mn-SOD

SOD2 (Superoxide Dismutase 2), also called IPO-B or MNSOD, is a mitochondrial matrix enzyme that scavenges oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions occurring in mitochondria. This gene is a member of the iron/manganese superoxide dismutase family. Using a somatic cell hybrid panel containing different segments of chromosome 6, they demonstrated that SOD2 is located in the region 6q25.3-qter which, together with the FISH analysis, indicated that SOD2 is in the distal portion of 6q25. The SOD2 gene encodes an intramitochondrial free radical scavenging enzyme that is the first line of defense against superoxide produced as a byproduct of oxidative phosphorylation. Adeno-associated viral delivery of the human SOD2 gene resulted in suppression of optic nerve degeneration and rescue of retinal ganglion cells. The findings suggested that reactive oxygen species contributed to retinal cell death and optic nerve damage in mice with complex I deficiency, and that expression of SOD2 attenuated the disease process.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry, 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human HepG2 whole cell lysates,
Lane 2: rat liver tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.

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Figure 2. IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).
SOD2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).
SOD2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 4. IHC analysis of SOD2 using anti-SOD2 antibody (PB9442).
SOD2 was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD2 Antibody (PB9442) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For SOD2 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.15.1.1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn SOD; Mn superoxide dismutase; MnSOD; MVCD6; SOD, Mitochodrial; SOD2; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial SOD2 GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD superoxide dismutase 2 superoxide dismutase [Mn], mitochondrial|Mn superoxide dismutase|epididymis secretory sperm binding protein|gastric cancer-associated lncRNA 1|indophenoloxidase B|manganese-containing superoxide dismutase|mangano-superoxide dismutase|superoxide dismutase 2, mitochondrial

For more info on SOD2, check out the SOD2 Infographic

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In this infographic, you will see the following information for SOD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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