Anti-Superoxide Dismutase 3/SOD3 Antibody

Product Name

Anti-Superoxide Dismutase 3/SOD3 Antibody

View all SOD3/EC-SOD Antibodies

SKU/Catalog Number

PA1899

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Superoxide Dismutase 3/SOD3 Antibody catalog # PA1899. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Superoxide Dismutase 3/SOD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1899)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SOD3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1899 is reactive to SOD3 in Human

Applications

PA1899 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

25.851kDa

Background of SOD3/EC-SOD

SOD3 (SUPEROXIDE DISMUTASE 3) also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is though to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry, 0.5-1μg/ml, Human,
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human,
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Anti-SOD3 antibody, PA1899, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: MCF-7 Cell Lysate

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Anti-SOD3 antibody, PA1899, IHC(F)
IHC(F): Human Placenta Tissue

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Figure 3. IHC analysis of SOD3 using anti-SOD3 antibody (PA1899).
SOD3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. ICC analysis of SOD3 using anti-SOD3 antibody (PA1899).
SOD3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For SOD3 (Source: Uniprot.org, NCBI)

Alternative Names

ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular SOD3 EC-SOD superoxide dismutase 3 extracellular superoxide dismutase [Cu-Zn]|superoxide dismutase 3, extracellular|testicular tissue protein Li 175

For more info on SOD3, check out the SOD3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SOD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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