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- Table of Contents
GAL · Western blot design guide
GAL Western Blot Planning Guide
Plan a GAL Western blot around the catalog-observed 21 kDa band, image-backed A00606-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
GAL Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 21 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Pituitary gland | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 21 kDa |
| ⓘ | Localization | Cytoplasm / Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Mouse / Rat |
Section 1
Real Curated GAL Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A00606-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected GAL Western Blot Band Size?
Use the product-observed 21 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 21 kDa | Matches the authoritative product WB observation. |
| 21 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected GAL appearancePlan around 21 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 21 kDa; use this as the primary experimental expectation. |
| Calculated mass | 21 kDa from UniProt Q8N6F7; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A00606-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 21 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing GAL band
A00606-1 · GAL
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for GAL Western blot
🧪Use Pituitary gland as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Pituitary gland (Medium)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for GAL
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Pituitary gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Hypothalamus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Small intestine | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Adrenal gland | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced GAL Western Blot Tips
Deeper troubleshooting and optimisation questions for GAL, answered from its protein features.
Which band should guide the blot?
Use 21 kDa, the observation attached to the authoritative A00606-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 21 kDa expectation.
Which positive control should I start with?
Start with Pituitary gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for GAL Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A00606-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
GAL Western Blot Reagents
Mouse/Rat-reactive GAL Western blot reagents with authoritative product imagery.
Real WB data
Anti-Galanin Antibody Picoband®
Cat # A00606-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q8N6F7
- Human Protein Atlas — GAL
- A00606-1 product record
- PMC5381108 — Involvement of galanin and galanin receptor 2 in nociceptive modulation in anterior cingulate cortex of normal rats and rats with mononeuropathy (Scientific reports, 2017)