Anti-APC Antibody

Boster Bio Anti-APC Antibody catalog # A00008. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00008
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

Product Name

Anti-APC Antibody

See all APC products

SKU/Catalog Number

A00008

Size

100μl

Form

Liquid

Description

Boster Bio Anti-APC Antibody catalog # A00008. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00008)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human APC. at AA range: 2770-2850

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00008 is reactive to APC in Human, Mouse, Rat

Applications

A00008 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000

Validation Images & Assay Conditions

Gene/Protein Information For APC (Source: Uniprot.Org, NCBI)

Uniprot ID

P25054

Gene ID

324

Gene Name

APC

Full Name

Adenomatous polyposis coli protein

Weight

311.646kDa

Superfamily

adenomatous polyposis coli (APC) family

Alternative Names

adenomatosis polyposis coli tumor suppressor; adenomatosis polyposis coli; adenomatous polyposis coli protein; adenomatous polyposis coli; APC; BTPS2; Deleted in polyposis 2.5; DP2; DP2.5DP2; DP3; FPC; GS; Min; Protein APC APC BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46 APC regulator of WNT signaling pathway adenomatous polyposis coli protein|APC, WNT signaling pathway regulator|WNT signaling pathway regulator|adenomatosis polyposis coli tumor suppressor|adenomatous polyposis coli (APC)|deleted in polyposis 2.5|epididymis secretory sperm binding protein|protein phosphatase 1, regulatory subunit 46|truncated adenomatosis polyposis coli

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on APC, check out the APC Infographic

APC infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for APC: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00008 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J, Sun Y, Gao X, Xiao H, Liu M. Plos One. 2013 Oct 23;8(10):E77829. Doi: 10.1371/Journal.Pone.0077829. Ecollection 2013. Down-Regulation Of Mir-129-5P Inhibits Growth And Induces Apoptosis In Laryngeal Squa...

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7 Customer Q&As for Anti-APC Antibody

Question

Will A00008 anti-APC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

As indicated on the product datasheet, A00008 anti-APC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-27

Question

I was wanting to use your anti-APC antibody for WB for human fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal brain identification?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

It shows on the product datasheet, A00008 anti-APC antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-17

Question

My question regarding product A00008, anti-APC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00008 anti-APC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-11

Question

Is this A00008 anti-APC antibody reactive to the isotypes of APC?

Verified Customer

Verified customer

Asked: 2019-04-11

Answer

The immunogen of A00008 anti-APC antibody is Synthesized peptide derived from the C-terminal region of human APC. at AA rangle: 2770-2850. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-04-11

Question

Would anti-APC antibody A00008 work on canine WB with pigmented layer of retina?

Verified Customer

Verified customer

Asked: 2017-12-28

Answer

Our lab technicians have not tested anti-APC antibody A00008 on canine. You can run a BLAST between canine and the immunogen sequence of anti-APC antibody A00008 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine pigmented layer of retina in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-28

Question

Is there a BSA free version of anti-APC antibody A00008 available?

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APC antibody A00008 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-10

Question

I see that the anti-APC antibody A00008 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-06-29

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-06-29

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00008
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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