Anti-Claudin 1 CLDN1 Antibody

Product Name

Anti-Claudin 1 CLDN1 Antibody

View all Claudin-1 Antibodies

SKU/Catalog Number

A01585

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Claudin 1 CLDN1 Antibody (Catalog # A01585). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Claudin 1 CLDN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01585)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Claudin 1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01585 is reactive to CLDN1 in Human, Mouse, Rat

Applications

A01585 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

22.744kDa

Background of Claudin-1

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (). CLDN1 acts as a coreceptor for HCV entry into hepatic cells.

Julie Dufresne and Daniel G. Cyr, Biol Reprod, May 2007; 76: 825 - 832.
Evemie Dub¨¦, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246.
Michele A. Battle, PNAS, May 2006; 103: 8419 - 8424.
Edmund C. Chang, Endocrinology, Oct 2006; 147: 4831 - 4842.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

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Western blot analysis of extracts from HeLa cells, using Claudin 1 antibody A01585.

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Western blot analysis of extracts from 293 cells (Lane 2), using Claudin 1 antiobdy A01585. The lane on the left is treated with synthesized peptide.

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Claudin 1 antibody A01585.

Gene/Protein Information For CLDN1 (Source: Uniprot.org, NCBI)

Alternative Names

claudin 1; Claudin1; Claudin-1; CLD1; CLDN1; ILVASCSenescence-associated epithelial membrane protein; SEMP1; SEMP1senescence-associated epithelial membrane protein 1 CLDN1 CLD1, ILVASC, SEMP1 claudin 1 claudin-1|senescence-associated epithelial membrane protein 1

For more info on CLDN1, check out the CLDN1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CLDN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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