Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated

Product Name

Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated

View all COL1A1 Antibodies

SKU/Catalog Number

B00329

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (Catalog # B00329). Tested in Dot blot, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at 4°C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-COLLAGEN Type I COL1A1 Antibody Biotin Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # B00329)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Collagen Type I from human and bovine placenta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

B00329 is reactive to COL1A1 in Human, Mouse, Rat

Applications

B00329 is guaranteed for Dot blot, Flow Cytometry Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

138.941kDa

Background of COL1A1

COLLAGEN Type I Antibody Biotin Conjugated is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:3,000 - 1:6,000
Flow Cytometry: User optimized
Fluorochrome Protein Value: 10-20
IHC: 1:50 - 1:200
IP: 1:100
WB: 1:3,000 - 1:6,000

Validation Images & Assay Conditions

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Western blot analysis of Collagen I expression in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (lane 1), PPARg-transduced cell lysates (lane 2). Load: 100 μg per lane. Collagen I at 138.9 kDa was detected using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 0.2 μg/10 ml. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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Collagen Type I was detected in paraffin-embedded sections of human skin at pH6 using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 10 μg/ml. The immunohistochemical sections were developed using SABC method (Catalog # SA1022). Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

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Collagen Type I was detected in paraffin-embedded sections of human skin at pH6 using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 10 μg/ml. The immunohistochemical sections were developed using SABC method (Catalog # SA1022). Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).

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Flow cytometry analysis of unstimulated mouse lung cells using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329). Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.

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Flow cytometry analysis of unstimulated primary adult human dermal fibroblast cells using biotin conjugated rabbit anti-Collagen Type I Antigen Affinity purified polyclonal antibody (Catalog # B00329) at 5

Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)

Alternative Names

Alpha-1 type I collagen; COL1A1; Collagen 1 alpha 1; Collagen 1; collagen alpha 1 chain type I; collagen alpha-1(I) chain; Collagen I alpha 1; collagen of skin, tendon and bone, alpha-1 chain; collagen, type I, alpha 1; Collagen1; Collagen-1; OI4; pro-alpha-1 collagen type 1 COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain

For more info on COL1A1, check out the COL1A1 Infographic

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In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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