Product Info Summary
SKU: | A00256-3 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone deacetylase 1 HDAC1 Antibody
SKU/Catalog Number
A00256-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Histone deacetylase 1 HDAC1 Antibody catalog # A00256-3. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone deacetylase 1 HDAC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00256-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 450-482 of Human HDAC-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00256-3 is reactive to HDAC1 in Human, Mouse, Rat
Applications
A00256-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
55.103kDa
Background of HDAC1
HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of HDAC1 using Anti-Histone deacetylase 1 HDAC1 Antibody (A00256-3).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC1 antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Histone deacetylase 1 HDAC1 Antibody A00256-3
Western blot analysis of HDAC1 on different lysates using anti-HDAC1 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For HDAC1 (Source: Uniprot.org, NCBI)
Gene Name
HDAC1
Full Name
Histone deacetylase 1
Weight
55.103kDa
Superfamily
histone deacetylase family
Alternative Names
EC 3.5.1.98; GON-10; HD1; HD1DKFZp686H12203; Histone Deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3L1; RPD3L1RPD3 HDAC1 GON-10, HD1, KDAC1, RPD3, RPD3L1 histone deacetylase 1 histone deacetylase 1|reduced potassium dependency, yeast homolog-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC1, check out the HDAC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone deacetylase 1 HDAC1 Antibody (A00256-3)
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No publications found for A00256-3
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Customer Reviews
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