Anti-sRANK Ligand TNFSF11 Antibody

Product Name

Anti-sRANK Ligand TNFSF11 Antibody

View all TRANCE/TNFSF11/RANK L Antibodies

SKU/Catalog Number

A00363-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-sRANK Ligand TNFSF11 Antibody (Catalog # A00363-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

sRANK Ligand antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-sRANK Ligand TNFSF11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00363-1)

Host

Rabbit

Contents

sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-sRANK-L antibody was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L. The immunogen is located within amino acids 140-190 of sRANK-L.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.

Reactive Species

A00363-1 is reactive to TNFSF11 in Human, Mouse, Rat

Applications

A00363-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

35.478kDa

Background of TRANCE/TNFSF11/RANK L

The receptor activator of NF-κB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 0.25-4 μg/mL; IHC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: sRANK-L A00363-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane.Antibodies: sRANK-L A00363-1 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation with Recombinant Protein
Loading: 30 ng of human sRANK-L recombinant protein per lane. Antibodies: sRANK-L A00363-1 (A: 1 μg/mL, B: 2 μg/mL and C: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 27kD.

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Western Blot Validation in Rat Liver Tissue
Loading: 15 μg of lysates per lane. Antibodies: sRANK-L A00363-1 (A: 0.25 μg/mL and B: 0.5 μg/mL)

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Immunohistochemistry Validation of sRANK-L in Human Liver Tissue
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti sRANK-L antibody (A00363-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Immunohistochemistry and Induced Expression Validation of sRANK-L in Rat Osteogenic Compartment (Hassan et al., 2017)
Protein analysis for sRANK-L by immunohistochemistry with anti-sRANK-L antibodies (A00363-1) in rat osteogenic cells. Strong immunoreactivity is shown for sRANK-L in osteogenic cells at 18hr after upper jaw molar extraction as compared to baseline.

Gene/Protein Information For TNFSF11 (Source: Uniprot.org, NCBI)

Alternative Names

CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 TNFSF11 CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf TNF superfamily member 11 tumor necrosis factor ligand superfamily member 11|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand 6B|tumor necrosis factor superfamily member 11

For more info on TNFSF11, check out the TNFSF11 Infographic

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In this infographic, you will see the following information for TNFSF11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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