Overview
| Product Name |
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
See all APP primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0658 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human APP with <100pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. APP tissue specificity: Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. . |
| Cite This Product |
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0658)
|
| ELISA Validation |
ELISA Validation Information
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Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<100pg/ml |
| Assay Range |
312pg/ml-20,000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: L18-L688 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For APP (Source: Uniprot.org, NCBI)
| Uniprot Id | P05067 |
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| NCBI Gene Id | 351 |
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| Species Of This Entry | Human |
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| Gene Name | APP |
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| Protein Name | Amyloid-beta precursor protein |
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| Superfamily | APP family |
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| Alternative Names | APP|Abeta; Amyloid beta; beta-Amyloid Peptide |
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| Molecular Weight | 86943 |
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| Post Tranlational Modifications | Cleavage; Phosphorylation; Glycosylation; Oxidation; Gamma-cleavage; Reduction; Biotinylation; Methylation; Dephosphorylation; Ubiquitination; Acetylation; Ribosylation; Sumoylation; Nitration; Demethylation; Deglycosylation; Myristoylation; Palmitoylation; Iodination; Deacetylation |
|---|
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. Only a minor proportion is present at the cell membrane; most of the protein is present in intracellular vesicles (PubMed:20580937). During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). Afte |
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| Gene Location | 21q21.3, on Chromasome 21, gene sequence: NC_000021.9 (25880550..26171128, complement) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For APP (Source: Uniprot.org, NCBI)
| Protein Function | Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER- dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. . |
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| Tissue Specificity | Expressed in all fetal tissues examined with highest levels in brain, kidney, heart and spleen. Weak expression in liver. In adult brain, highest expression found in the frontal lobe of the cortex and in the anterior perisylvian cortex- opercular gyri. Moderate expression in the cerebellar cortex, the posterior perisylvian cortex-opercular gyri and the temporal associated cortex. Weak expression found in the striate, extra- striate and motor cortices. Expressed in cerebrospinal fluid, and plasma. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in non- neuronal cells. Isoform APP751 is the most abundant form in T- lymphocytes. Appican is expressed in astrocytes. |
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| Research Areas | Alzheimers Research; Apoptosis; Cell Cycle and Replication; Neurodegeneration; Neuronal Cell Markers; Neuroscience; Signal Transduction |
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| Related Diseases | Alzheimer's Disease; Plaque, Amyloid; Senile Plaques; Dental Plaque; Dementia; Nerve Degeneration; Neurodegenerative Disorders; Neurofibrillary Tangles; Neuroblastoma; Alzheimer Disease, Familial; Inflammation; Impaired Cognition; Nervousness; Down Syndrome; Amyloid Deposition; Memory Impairment; Neuropathology Disease; Amyloidosis; Neurotoxicity Syndromes; Brain Injuries |
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| Related Pathways | Pathogenesis; Secretion; Proteolysis; Transport; Aging; Localization; Cell Death; Protein Processing; Cognition; Endocytosis; Secretory Pathway; Hyperphosphorylation; Translation; Neurogenesis; Inflammatory Response; Neuroprotection; Cell Adhesion; Synaptic Transmission; Glycosylation; Rna Interference |
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| Background | Amyloid precursor protein(APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid(Abeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive. |
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| Scientific References | PMID: 2881207 by Kang J., et al. The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor.
PMID: 2893289 by Ponte P., et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. |
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Kit Components And Assay QC Data Details Of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0658-CAP | 96-well plate precoated with anti-Human APP antibody | 1 |
| EK0658-ST | lyophilized recombinant Human APP standard | 20ng/tube |
| EK0658-DA | biotinylated anti-Human APP antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0658 Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.130 | 0.181 | 0.221 | 0.323 | 0.506 | 0.788 | 1.248 | 1.854 |
We measured random samples of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 775 | 2619 | 9426 | 755 | 2868 | 9446 |
| Standard deviation | 50.37 | 188.56 | 377.04 | 54.36 | 226.57 | 510.08 |
| CV(%) | 6.5% | 7.2% | 4% | 7.2% | 7.9% | 5.4% |
We measured the performance consistency of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 775 | 742 | 738 | 700 | 738 | 26.58 | 3.6% |
| Sample 2 | 2619 | 3033 | 2635 | 3053 | 2835 | 208.19 | 7.3% |
| Sample 3 | 9426 | 9494 | 8945 | 9504 | 9342 | 231.3 | 2.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 3
| Zawilla Nh, Taha Fm, Kishk Na, Farahat Sa, Farghaly M, Hussein M. J Inorg Biochem. 2014 Oct;139:57-64. Doi: 10.1016/J.Jinorgbio.2014.06.003. Epub 2014 Jun 11. Occupational Exposure To Aluminum And Its Amyloidogenic Link With Cognitive Functions. |
PubMed ID 24973993 |
| Nie J, Luo Y, Huang Xn, Gong Qh, Wu Q, Shi Js. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. Doi: 10.1016/J.Ejphar.2009.09.039. Epub 2009 Sep 24. Icariin Inhibits Beta-Amyloid Peptide Segment 25-35 Induced Expression Of Beta-Secretase In Rat Hippoc... |
PubMed ID 19782061 |
| Effect of general anesthesia on serum ?-amyloid protein and regional cerebral oxygen saturation of elderly patients after subtotal gastrectomy |
PubMed ID 28101151 |
