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Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 100pg/ml. Cited in 3 publication(s).
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ Product Summary
| SKU: | EK0658 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10 pg/ml |
| Assay Range: | 312 pg/ml - 20,000 pg/ml |
Overview
| Product Name | Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
See more APP products |
|---|---|
| Catalog# | EK0658 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 100pg/ml. |
| Cite This Product | Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0658) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10 pg/ml |
| Assay Range | 312 pg/ml - 20,000 pg/ml |
| Immunogen/Standard | Expression system for standard: NS0; Immunogen sequence: L18-L688 |
| Antibody Clonalities | Capture antibody|Detection antibody polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For APP (Source: Uniprot.org, NCBI)
| Uniprot Id | P05067 |
|---|---|
| NCBI Gene Id | 351 |
| Species Of This Entry | Human |
| Gene Name | APP |
| Protein Name | Amyloid-beta precursor protein |
| Superfamily | APP family |
| Alternative Names | APP|Abeta; Amyloid beta; beta-Amyloid Peptide |
| Molecular Weight | 86943 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on APP, check out the APP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for APP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0658-CAP | Anti-Human APP Pre-coated 96-well strip microplate | 1 |
| EK0658-ST | Human APP Standard | 2 vials, 20 ng/tube |
| EK0658-DA | Human APP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0658 Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.130 | 0.181 | 0.221 | 0.323 | 0.506 | 0.788 | 1.248 | 1.854 |
We measured random samples of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 775 | 2619 | 9426 | 755 | 2868 | 9446 |
| Standard deviation | 50.37 | 188.56 | 377.04 | 54.36 | 226.57 | 510.08 |
| CV (%) | 6.5% | 7.2% | 4% | 7.2% | 7.9% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of APP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 775 | 742 | 738 | 700 | 738 | 26.58 | 3.6% |
| Sample 2 | 2619 | 3033 | 2635 | 3053 | 2835 | 208.19 | 7.3% |
| Sample 3 | 9426 | 9494 | 8945 | 9504 | 9342 | 231.3 | 2.4% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 3
| Effect of general anesthesia on serum ?-amyloid protein and regional cerebral oxygen saturation of elderly patients after subtotal gastrectomy | PubMed ID 28101151 |
| Nie J, Luo Y, Huang Xn, Gong Qh, Wu Q, Shi Js. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. Doi: 10.1016/J.Ejphar.2009.09.039. Epub 2009 Sep 24. Icariin Inhibits Beta-Amyloid Peptide Segment 25-35 Induced Expression Of Beta-Secretase In Rat Hippoc... | PubMed ID 19782061 |
| Zawilla Nh, Taha Fm, Kishk Na, Farahat Sa, Farghaly M, Hussein M. J Inorg Biochem. 2014 Oct;139:57-64. Doi: 10.1016/J.Jinorgbio.2014.06.003. Epub 2014 Jun 11. Occupational Exposure To Aluminum And Its Amyloidogenic Link With Cognitive Functions. | PubMed ID 24973993 |
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Questions and answers from customers related to EK0658 Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™
8 Related Questions
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2019-09-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-08
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of APP in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
R. Li
Asked: 2019-06-27
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the APP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis APP using the EK0658 Human APP Picokine® ELISA Kit?
Verified Customer
Asked: 2019-06-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-06-25
Question
Q: how do I analyze ELISA data? I have performed APP level in plasma.
L. Edwards
Asked: 2018-11-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-11-05
Question
Q: What is the optimal O.D. value for APP ELISA kit? I used your APP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain APP even though the O.D. values are not very high?
Verified Customer
Asked: 2016-05-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this APP ELISA kit to have sensitivity of 100pg/ml, that means the minimum amount of APP that can be declared/interpreted as positive by the above standard is 100pg/ml.
Boster Scientific Support
Answered: 2016-05-07
Question
Q: if the enzyme conjugated APP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the APP antigen?
R. Chen
Asked: 2014-05-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Wang
Asked: 2014-04-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-04-09
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| (888) 466-3604 |
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