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Product Info Summary
| SKU: | EK0658 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine®
SKU/Catalog Number
EK0658
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 100pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human APP / Amyloid Precursor Protein ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0658)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: L18-L688
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0658 is reactive to APP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0658 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of APP
Amyloid precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. Its primary function is not known, though it has been implicated as a regulator of synapse formation, neural plasticity and iron export. APP is best known and most commonly studied as the precursor molecule whose proteolysis generates beta amyloid (Abeta), a 39- to 42-amino acid peptide whose amyloid fibrillar form is the primary component of amyloid plaques found in the brains of Alzheimer's disease patients. APP undergoes posttranslational proteolytic processing by alpha-, beta-, and gamma-secretases. Alpha-secretase generates soluble amyloid protein, while beta- and gamma-secretases generate APP components with amyloidogenic features. These 2 processing pathways are mutually exclusive.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0658-CAP | Anti-Human APP Pre-coated 96-well strip microplate | 1 |
| EK0658-ST | Human APP Standard | 2 vials, 20 ng/tube |
| EK0658-DA | Human APP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 200000000 | 20000000 | 2000000 | 200000 | 20000 |
| O.D. | 0.130 | 0.181 | 0.221 | 0.323 | 0.506 | 0.788 | 1.248 | 1.854 |
Data Example Images
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Human APP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect APP, Dilution ratio of 1:100, concentration in serum is around 400ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 775 | 2619 | 9426 | 755 | 2868 | 9446 |
| Standard deviation | 50.37 | 188.56 | 377.04 | 54.36 | 226.57 | 510.08 |
| CV (%) | 6.5% | 7.2% | 4% | 7.2% | 7.9% | 5.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of APP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 775 | 742 | 738 | 700 | 738 | 26.58 | 3.6% |
| Sample 2 | 2619 | 3033 | 2635 | 3053 | 2835 | 208.19 | 7.3% |
| Sample 3 | 9426 | 9494 | 8945 | 9504 | 9342 | 231.3 | 2.4% |
Protein Target Info & Infographic
Gene/Protein Information For APP (Source: Uniprot.Org, NCBI)
Uniprot ID
P05067
Gene ID
351
Gene Name
APP
Full Name
Amyloid-beta precursor protein
Weight
86.943kDa
Superfamily
APP family
Alternative Names
Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on APP, check out the APP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for APP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human APP / Amyloid Precursor Protein ELISA Kit PicoKine® (EK0658)
EK0658 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of general anesthesia on serum ?-amyloid protein and regional cerebral oxygen saturation of elderly patients after subtotal gastrectomy
Nie J, Luo Y, Huang Xn, Gong Qh, Wu Q, Shi Js. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. Doi: 10.1016/J.Ejphar.2009.09.039. Epub 2009 Sep 24. Icariin Inhibits Beta-Amyloid Peptide Segment 25-35 Induced Expression Of Beta-Secretase In Rat Hippoc...
Zawilla Nh, Taha Fm, Kishk Na, Farahat Sa, Farghaly M, Hussein M. J Inorg Biochem. 2014 Oct;139:57-64. Doi: 10.1016/J.Jinorgbio.2014.06.003. Epub 2014 Jun 11. Occupational Exposure To Aluminum And Its Amyloidogenic Link With Cognitive Functions.
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
14 Customer Q&As for Human APP / Amyloid Precursor Protein ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for APP ELISA after freezing?
J. Liu
Verified customer
Asked: 2020-11-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test APP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-11-18
Question
Q: Can APP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Wu
Verified customer
Asked: 2020-02-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-02-14
Question
Does the Human APP PicoKine™ ELISA Kit detect all of APP, APP40 and APP42?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The Human APP PicoKine™ ELISA Kit detects extracellular APP with the immunogen sequence L18-L688.
Boster Scientific Support
Answered: 2019-11-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-09-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-09-08
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of APP in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
R. Li
Verified customer
Asked: 2019-06-27
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the APP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis APP using the EK0658 Human APP Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-06-25
Question
Q: can you recommend the dilution ratio of serum samples for detection of APP in Monkey plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the APP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-03-06
Question
Q: how do I analyze ELISA data? I have performed APP level in plasma.
L. Edwards
Verified customer
Asked: 2018-11-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-11-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Y. Huang
Verified customer
Asked: 2018-10-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-14
Question
Q: can I use citrate plasma as samples in Monkey APP Picokine® ELISA Kit (Catalog # EK0658-PR)?
K. Parker
Verified customer
Asked: 2018-10-12
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of APP causing disruption of its protein structure. APP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the APP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-12
Question
Q: how do I analyze ELISA data? I have performed APP level in plasma.
Verified Customer
Verified customer
Asked: 2016-10-13
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-10-13
Question
Q: What is the optimal O.D. value for APP ELISA kit? I used your APP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain APP even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-05-07
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this APP ELISA kit to have sensitivity of 100pg/ml, that means the minimum amount of APP that can be declared/interpreted as positive by the above standard is 100pg/ml.
Boster Scientific Support
Answered: 2016-05-07
Question
Q: if the enzyme conjugated APP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the APP antigen?
R. Chen
Verified customer
Asked: 2014-05-01
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Wang
Verified customer
Asked: 2014-04-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-04-09
