Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™

Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 100pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0658
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™

See all APP products

SKU/Catalog Number

EK0658

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (96 Tests). Quantitate Human APP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 100pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0658)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L18-L688

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0658 is reactive to APP in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0658 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0658-CAP Anti-Human APP Pre-coated 96-well strip microplate 1
EK0658-ST Human APP Standard 2 vials, 20 ng/tube
EK0658-DA Human APP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031262520000000020000000200000020000020000
O.D.0.1300.1810.2210.3230.5060.7881.2481.854

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect APP, Dilution ratio of 1:100, concentration in serum is around 400ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7752619942675528689446
Standard deviation50.37188.56377.0454.36226.57510.08
CV (%)6.5%7.2%4%7.2%7.9%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of APP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177574273870073826.583.6%
Sample 226193033263530532835208.197.3%
Sample 394269494894595049342231.32.4%
*number of samples for each test n=16.

Gene/Protein Information For APP (Source: Uniprot.Org, NCBI)

Uniprot ID

P05067

Gene ID

351

Gene Name

APP

Full Name

Amyloid-beta precursor protein

Weight

86.943kDa

Superfamily

APP family

Alternative Names

Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on APP, check out the APP Infographic

APP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for APP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0658 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of general anesthesia on serum ?-amyloid protein and regional cerebral oxygen saturation of elderly patients after subtotal gastrectomy

Nie J, Luo Y, Huang Xn, Gong Qh, Wu Q, Shi Js. Eur J Pharmacol. 2010 Jan 25;626(2-3):213-8. Doi: 10.1016/J.Ejphar.2009.09.039. Epub 2009 Sep 24. Icariin Inhibits Beta-Amyloid Peptide Segment 25-35 Induced Expression Of Beta-Secretase In Rat Hippoc...

Zawilla Nh, Taha Fm, Kishk Na, Farahat Sa, Farghaly M, Hussein M. J Inorg Biochem. 2014 Oct;139:57-64. Doi: 10.1016/J.Jinorgbio.2014.06.003. Epub 2014 Jun 11. Occupational Exposure To Aluminum And Its Amyloidogenic Link With Cognitive Functions.

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8 Customer Q&As for Human APP / Amyloid Precursor Protein ELISA Kit PicoKine™

Question

Does the Human APP PicoKine™ ELISA Kit detect all of APP, APP40 and APP42?

Verified Customer

Verified customer

Asked: 2019-11-05

Answer

The Human APP PicoKine™ ELISA Kit detects extracellular APP with the immunogen sequence L18-L688.

Boster Scientific Support

Answered: 2019-11-05

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-09-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-09-08

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of APP in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

R. Li

Verified customer

Asked: 2019-06-27

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the APP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-27

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis APP using the EK0658 Human APP Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-06-25

Question

Q: how do I analyze ELISA data? I have performed APP level in plasma.

L. Edwards

Verified customer

Asked: 2018-11-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-11-05

Question

Q: What is the optimal O.D. value for APP ELISA kit? I used your APP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain APP even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-05-07

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this APP ELISA kit to have sensitivity of 100pg/ml, that means the minimum amount of APP that can be declared/interpreted as positive by the above standard is 100pg/ml.

Boster Scientific Support

Answered: 2016-05-07

Question

Q: if the enzyme conjugated APP antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the APP antigen?

R. Chen

Verified customer

Asked: 2014-05-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-05-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Wang

Verified customer

Asked: 2014-04-09

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-04-09

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