Product Info Summary
SKU: | M01263-2 |
---|---|
Size: | 100uL |
Reactive Species: | All species |
Host: | Rabbit |
Application: | ChIP, IP, IHC, ICC, WB |
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Product info
Product Name
Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112
View all beta-Actin Antibodies
SKU/Catalog Number
M01263-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (Catalog # M01263-2). Tested in WB, IP, ChIP, ICC, IHC applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM112
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of beta-actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to β-Actin (Actin, cytoplasmic 1). No cross-reactivity with other isoforms of Actin.
Reactive Species
M01263-2 is reactive to ACTB in All species
Applications
M01263-2 is guaranteed for ChIP, IP, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.737kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (WB): 1:1000 dilution
Immunoprecipitation (IP): 1:200 dilution
Chromatin IP (ChIP): 1:200 dilution
Immunocytochemistry (ICC): 1:200 dilution
Immunohistochemistry (IHC): 1:200 dilution.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western Blotting result
Western blot of A431 cells, using Clone RM112 at 1/1000 dilution. A -Actin band showed at the predicted MW (42 kDa).
Click image to see more details
Figure 2. ICC result
Immunocytochemical staining of HeLa cells, using anti--Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue)
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41.737kDa
Superfamily
actin family
Alternative Names
ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (M01263-2)
Hello CJ!
M01263-2 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
TMEM16A ameliorates vascular remodeling by suppressing autophagy via inhibiting Bcl-2-p62 complex formation
Integrated bioinformatics analyses of key genes involved in hepatocellular carcinoma immunosuppression
CaSR activates PKCδ to induce cardiomyocyte apoptosis via ER stress‑associated apoptotic pathways during ischemia/reperfusion
Gastro-Protective Effects of Albizia anthelmintica Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies
Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma
COLEC12 regulates apoptosis of osteosarcoma through Toll‐like receptor 4–activated inflammation
CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma
CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma
CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma
Yang H,Lu Z,Huo C,Chen Y,Cao H,Xie P,Zhou H,Liu D,Liu J,Yu L.Liang-Ge-San, a Classic Traditional Chinese Medicine Formula, Attenuates Lipopolysaccharide-Induced Acute Lung Injury Through Up-Regulating miR-21.Front Pharmacol.2019 Nov 14;10:1332.doi:10.3389/fphar.2019.01332.PMID:31803051;PMCID:PMC6868520.
Species: Mouse
M01263-2 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA
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