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Product Info Summary
| SKU: | M01263-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | All species |
| Host: | Rabbit |
| Application: | ChIP, IP, IHC, ICC, WB |
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Product info
Product Name
Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112
SKU/Catalog Number
M01263-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (Catalog # M01263-2). Tested in WB, IP, ChIP, ICC, IHC applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM112
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of beta-actin
Cross-reactivity
This antibody reacts to β-Actin (Actin, cytoplasmic 1). No cross-reactivity with other isoforms of Actin.
Reactive Species
M01263-2 is reactive to ACTB in All species
Calculated molecular weight
41.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01263-2 is guaranteed for ChIP, IP, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (WB): 1:1000 dilution
Immunoprecipitation (IP): 1:200 dilution
Chromatin IP (ChIP): 1:200 dilution
Immunocytochemistry (ICC): 1:200 dilution
Immunohistochemistry (IHC): 1:200 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western blot of A431 cells, using Clone RM112 at 1/1000 dilution. A -Actin band showed at the predicted MW (42 kDa).
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A ASCL2 expressed and B quantificated in different tissues. C IHC for ASCL2 (× 100). D ASCL2 expressed and E quantificated in different cell lines. Protein levels were normalized to β-actin (tumor vs. para-tumor, and MGC803 vs. other cells, n = 6, * P < 0.05)
Index in PubMed under a CC BY license. PMID: 36008864
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ICC result
Immunocytochemical staining of HeLa cells, using anti--Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue)
Specific Publications For Anti-beta -Actin ACTB Rabbit Monoclonal Antibody, Clone#RM112 (M01263-2)
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