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- Table of Contents
Facts about Galanin peptides.
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Mouse | |
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Gene Name: | Gal |
Uniprot: | P47212 |
Entrez: | 14419 |
Belongs to: |
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galanin family |
GAL; GAL1; galanin prepropeptide; Galanin; GALN; GALNgalanin-related peptide; GLNNgalanin-message-associated peptide; GMAP; MGC40167
Mass (kDA):
13.471 kDA
Mouse | |
---|---|
Location: | 19 A|19 3.16 cM |
Sequence: | 19; |
Boster Bio's GAL Marker is a versatile product that allows scientists to run a variety of tests with minimum effort. This tool is a great choice to scientists who are conducting tests on species or applications. In addition to its versatility, it also gives scientists the possibility of submitting results for crediting product credits, a feature that is applicable to all scientists around the globe. Here are a few of most frequently used applications of the GAL Marker.
The GAL marker is a commonly used surrogate for p16Ink4a, an end-effector for cell cycle stoppage. Although this surrogate has some limitations, it is effective in detecting cell senescence. This marker is sensitive however it has a high false-positive rate because it could show a false negative response in T cells and maturing macrophages of the tissue. The GAL Marker can be used together with the p16Ink4a marker, which is an oxygenative status that is not specific or both.
Boster Bio In situ hybridization detection kits are designed to detect specific DNA sequences in cells and tissues. It detects a specific IL6 gene that is located on 7p15 of the chromosome. The kit comes with the rat antiIL6 peptide an unlabeled complement probe and buffer. The samples were incubated in 37degC for 1 hour, and following that, they were washed in in situ hybridization PBS for five minutes. DAB was used to produce the colour. The samples were used both as negative and control. Under light microscopy, positive signals were seen.
Boster Bio is a leading manufacturer of antibodies. It is a leading manufacturer of ELISA kits that are sensitive to the picogram and IHC-optimized. They also offer polyclonal. Boster Bio's extensive portfolio contains more than 12,000 antibodies. They have been validated against a panel comprising 250 human tissues and untransfected cells. All Boster antibodies are subject to rigorous quality control and are supported by outstanding customer service.
Single-B cell screening is an effective method of identifying high-affinity antibodies against a specific antigen in just a few weeks. Sorting single cells is a powerful method of identifying antibodies from viable B cell derived from convalescent patients or immunized animals. This process involves high-throughput single cell sorting as well as the recovery of paired VH/VL antibody genes, as well as expression and evaluation of the potential antibodies.
Immunoassays were performed using these primary antibodies to detect influenza. Flow cytometry was employed to determine the affinity of the isolated antibodies to biotinylated SARS-CoV-2 RBD immobilized on magnetic beads. Three independent tests were used to determine the half-maximal concentrations. The mean values are shown. In the previous study, Boster Bio compared four antibodies against one another. The IC50 values of 13I1 as well as 12H2 were similar. However, CB6 was significantly improved from the earlier studies.
The binding capability of 13I1 antibody was significantly diminished against the South African and B.1.351 variations. These results are in line with previously reported antibodies and nanobodies, suggesting that competing antibodies are competing for the same RBD and provide useful information for the creation of future broadly neutralizing antibodies. And because the antibody's binding affinity is high, it has low background. This is crucial for doctors and researchers working on infectious diseases.
The staining of XGal enzymes and IHC workflow that utilizes the GAL marker are complementary tools that confirm the expression of ssGal. When cells express the GAL marker, X-Gal develops into an intense blue precipitant. However, enzyme activity is rapidly depleted over time and must be sent for analysis within 48 hours of perfusion. In addition, this procedure requires trained personnel and expensive equipment.
PMID: 8982069 by Kofler B., et al. Molecular cloning and characterisation of the mouse preprogalanin gene.
PMID: 8614559 by Lundkvist J., et al. cDNA sequence, ligand binding, and regulation of galanin/GMAP in mouse brain.
*More publications can be found for each product on its corresponding product page