Browse PicoKine® ELISA Kits

ELISA Fundamental Principle, How ELISA Works

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface...

ELISA Sample Preparation Guide

The procedure below provides a general guidance for the preparation of commonly tested samples for use in ELISA assays. Please check with the literature for experiments similar to yours for your new assay development. In this article...

An ELISA Guide That You Cannot Miss

This guide will teach you everything you need to become an ELISA expert, including critical review of principle, all-in-one FAQs, and more...

Boster's Free Troubleshooting Guides

You are at the right place and at the right time for our latest series of troubleshooting handbooks. They are available at your finger tips with just a few clicks...

Guidelines for Preparing ELISA Standards

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay used to detect the concentration of a specific protein in a liquid sample. Three different types of data output can be obtained...

Four Types of ELISA Assay

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats illustrated below, where differences in...

ELISA Troubleshooting: Weak Signal

Weak or no color development in an ELISA assay can indicate that the target protein is present in minute quantities in the sample, if at all. It can also mean that there is something wrong with the assay or the reagents that prevents efficient detection....

ELISA Blocking Optimization

The process of coating an ELISA plate relies on the passive binding activity of the solid phase, which immobilizes biomolecules on the well surface. Without appropriate blocking, the plate would bind the detection antibody alongside the antigen or detection...

ELISA Data Analysis

After performing an ELISA with a ready-to-use ELISA kit or an antibody pair kit, the data generated must be analyzed to quantitate the target protein concentrations. We discuss the different aspects to consider for more consistent and accurate ELISA data. Furthermore, we provide a step-by-step guide to create the standard curve for analysis.....

Pitfalls to Avoid for ELISA!

ELISA (enzyme-linked immunosorbent assay) is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages, there are some mistakes that could turn your ELISA experiment sour...

ELISA Blocking Buffer Optimization

There are a variety of blocking buffers, not one of which is ideal for every combination of plate type, assay format, and detection system. Every blocking buffer represents a compromise between....

How to Perfect Your ELISA Standards: Protocol and Troubleshooting

At Boster, one common question we get from researchers is, “How do I prepare the ELISA standard?” We’re glad you asked because proper construction of the standard curve is the very first step....