Research Spotlight – March 2022

The March edition of Research Spotlight presents 10 recent publications from scientists whom Boster has served.

In this edition, scientists discussed how a better understanding of the PD-L1 expression regulation can be pivotal in achieving better clinical outcomes in EBV-infected cancer patients. Another research study proposed a self-powered smart patch that serves as a reservoir of chemokine-/neural-directing exosomes, offering a serviceable strategy to stimulate skin neuro-engineering and regeneration of tissues and organs.

In terms of bone diseases, one paper revealed the potential role of Calca, an osteoblast-derived ProCT in the bone microenvironment that is required to limit bone resorption during iPTH. Several development strategies in the fields of nanotechnology and biomaterials were also achieved through the construction of cell-responsive peptide assemblies and nanorod-like coating with ions doping.

Explore below for a closer look at these research discoveries!

EBV miRNAs BART11 and BART17-3p Promote Immune Escape Through the Enhancer-Mediated Transcription of PD-L1

Authors: Wang, J., Ge, J., Wang, Y., Xiong, F., Guo, J., Jiang, X., Zhang, L…
Journal: Nature Communications

Epstein-Barr virus (EBV) is reportedly the first identified human tumor virus, and is closely related to the occurrence and development of nasopharyngeal carcinoma (NPC), gastric carcinoma (GC), and several lymphomas. PD-L1 expression is elevated in EBV-positive NPC and GC tissues; however, the specific mechanisms underlying the EBV-...

Cited Boster Product(s): Mouse IL-2 ELISA Kit PicoKine® (EK0398); Mouse Granzyme B ELISA Kit PicoKine® (EK1115); Mouse IFN Gamma / IFNG / Interferon Gamma ELISA Kit PicoKine® (EK0375)

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα

Authors: Feng, L., Li, C., Zeng, L.W., Gao, D., Sun, Y.H., Zhong, L., Lin, H…
Journal: Signal Transduction and Targeted Therapy

Interleukin-3 (IL-3) is a hematopoietic growth factor and critical regulator of inflammatory response such as sepsis. IL-3 binds to IL-3 receptor α (IL-3Rα), which is then associated with IL-3Rβ to initiate signaling. How IL-3-triggered physiological and pathological effects are regulated at the receptor level is unclear. Here, we...

Cited Boster Product(s): Mouse IL-1 Beta ELISA Kit PicoKine® (EK0394)

Procalcitonin is Expressed in Osteoblasts and Limits Bone Resorption Through Inhibition of Macrophage Migration During Intermittent PTH Treatment

Authors: Baranowsky, A., Jahn, D., Jiang, S., Yorgan, T., Ludewig, P...
Journal: Bone Research

Intermittent injections of parathyroid hormone (iPTH) are applied clinically to stimulate bone formation by osteoblasts, although continuous elevation of parathyroid hormone (PTH) primarily results in increased bone resorption. Here, we identified Calca, encoding the sepsis biomarker procalcitonin (ProCT), as a novel target gene of...

Cited Boster Product(s): Anti-Macrosialin CD68 Antibody (PA1518)

Cubic Multi-Ions-Doped Na2TiO3 Nanorod-Like Coatings: Structure-Stable, Highly Efficient Platform for Ions-Exchanged Release to Immunomodulatory Promotion on Vascularized Bone Apposition

Authors: Yu, D., Li, B., Yu, M., Guo, S., Guo, Z., Han, Y.
Journal: Bioactive Materials

The dissolution-derived release of bioactive ions from ceramic coatings on metallic implants, despite improving osseointegration, renders a concern on the interfacial breakdown of the metal/coating/bone system during long-term service. Consequently, persistent efforts to seek alternative strategies instead of dissolution-derived...

Cited Boster Product(s): Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®488 Conjugated (BA1127); Goat Anti-Mouse IgG (H+L) Secondary Antibody, DyLight®594 Conjugated (BA1141)

Enzymatic Self-Assembly Nanofibers Anchoring Mesenchymal Stem Cells Induce Cell Spheroids and Amplify Paracrine Function for Myocardial Infarction Therapy

Authors: Fan, X., Zhan, J., Pan, X., Liao, X., Guo, W., Chen, P., Li, H., Feng, W...
Journal: Chemical Engineering Journal

Transplantation of stem cells with biomaterials has proved to be an effective way to improve cell engraftment and survival in treating degenerative diseases. Owing to their structural resemblance to the extracellular matrix (ECM), synthetic biomaterials have been shown to mimic the native tissue microenvironment and support regeneration...

Cited Boster Product(s): Anti-Fibronectin Rabbit Monoclonal Antibody (M00564-3); Anti-Collagen III Rabbit Monoclonal Antibody (M00788-1)

Self-Powered Smart Patch Promotes Skin Nerve Regeneration and Sensation Restoration by Delivering Biological-Electrical Signals in Program

Authors: Tan, M.H., Xu, X.H., Yuan, T.J., Hou, X., Wang, J., Jiang, Z.H…
Journal: Biomaterials

Skin wound is always accompanied with nerve destruction. Due to the limited clinical treatment option, loss of skin sensation with unsatisfactory nerve regeneration is remained to be a challenge for wound therapy. Endogenous mesenchymal stem cells (MSCs) based in situ regeneration, of which, MSCs recruited by chemokines and directed...

Cited Boster Product(s): Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Cy3 Conjugated (BA1032); Goat Anti-Mouse IgG (H+L) Secondary Antibody, FITC Conjugated (BA1101)

LncRNA AY Promotes Hepatocellular Carcinoma Metastasis by Stimulating ITGAV Transcription

Authors: Kang, C.L., Qi, B., Cai, Q.Q., Fu, L.S., Yang, Y., Tang, C., Zhu, P...
Journal: Theranostics

Rationale: Tumor metastasis is the main cause for cancer-related death. However, the driving molecules of metastasis remain largely unknown. Here, we aim to identify long non-coding RNAs (lncRNAs) critical for human hepatocellular carcinoma (HCC) metastasis. Methods: Microarrays were used to screen a comprehensive set of lncRNAs with...

Cited Boster Product(s): ISH Detection Kit (POD) (MK1030)

Autophagy-Based Unconventional Secretion of HMGB1 in Glioblastoma Promotes Chemosensitivity to Temozolomide Through Macrophage M1-Like Polarization

Authors: Li, Z., Fu, W.J., Chen, X.Q., Wang, S., Deng, R.S., Tang, X.P...
Journal: Journal of Experimental & Clinical Cancer Research

Background: Glioblastoma (GB) is the most common and highly malignant brain tumor characterized by aggressive growth and resistance to alkylating chemotherapy. Autophagy induction is one of the hallmark effects of anti-GB therapies with temozolomide (TMZ). However, the non-classical form of autophagy, autophagy-based unconventional...

HOXA-AS2 Contributes to Regulatory T Cell Proliferation and Immune Tolerance in Glioma Through the miR-302a/KDM2A/JAG1 Axis

Authors: Zhong, C., Tao, B., Li, X., Xiang, W., Peng, L., Peng, T., Chen, L...
Journal: Cell Death & Disease

Long non-coding RNAs (lncRNAs) have been manifested to manipulate diverse biological processes, including tumor-induced immune tolerance. Thus, we aimed in this study to identify the expression pattern of lncRNA homeobox A cluster antisense RNA 2 (HOXA-AS2) in glioma and decipher its role in immune tolerance and glioma progression. We...

Cited Boster Product(s): RIPA Lysis Buffer (AR0105-100); BCA Protein Assay Kit (AR0146)

Deacetylation of Caveolin-1 by Sirt6 Induces Autophagy and Retards High Glucose-Stimulated LDL Transcytosis and Atherosclerosis Formation

Authors: Zhao, Y., Jia, X., Yang, X., Bai, X., Lu, Y., Zhu, L., Cheng, W…
Journal: Metabolism

Background: Atherosclerosis (AS) is the basis of diabetic macrovascular complications. The plasma low-density lipoprotein (LDL) particles transcytosis across endothelial cells (ECs) and deposition under the endothelium is the initiation step of AS. We previously reported that high glucose inhibits the autophagic degradation of...

Cited Boster Product(s): 5nm Colloidal Gold Labeled Secondary Antibody, Goat Anti-Mouse IgG (H+L) (GA1003)

Osteogenic Differentiation of Mesenchymal Stem Cells Promotes c-Jun-Dependent Secretion of Interleukin 8 and Mediates the Migration and Differentiation of CD4+ T Cells

Authors: Ye, F., Li, J., Xu, P., Xie, Z., Zheng, G., Liu, W., Ye, G., Yu, W., Lin, J...
Journal: Stem Cell Research & Therapy

The immune system and the skeletal system have complex interactions in the bone marrow and even in the joints, which has promoted the development of the concept of osteoimmunology. Some evidence has indicated that T cells and B cells contribute to the balance between the resorption and formation of bone. However, there...

Cited Boster Product(s): HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054); HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (BA1050)