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Product Info Summary
| SKU: | EK7001 |
|---|---|
| Size: | 96wells/kit |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | Serum |
Product info
Product Name
Human Proinsulin ELISA Kit
SKU/Catalog Number
EK7001
Size
96wells/kit
*Question: How many samples can I assay/run in this kit?
Description
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Proinsulin. 96wells/kit, with removable strips.
Storage & Handling
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Proinsulin ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7001)
Clonality of Antibodies
See Datasheet for details
Sensitivity
2.6 pmol/L
Assay Range
2.6-66 pmol/L
Cross-Reactivity
There is no detectable cross-reactivity.
Reactive Species
EK7001 is reactive to in Human samples
Validated Sample Types
Serum
Application Guarantee
EK7001 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Description | Quantity |
| Microwell coated with anti Pro-insulin Antibody | 12x8x1 Microwells |
| Proinsulin Standards | 6 vials (ready to use) 1ml |
| Pro-Insulin Enzyme Conjugate 11X | 1 vial 1.2 ml |
| TMB Substrate | 1 bottle (ready to use) 14ml |
| Stop Solution | 1 bottle (ready to use) 14ml |
| Wash concentrate 40X | 1 bottle (ready to use) 30ml |
| Sample Diluent | 1 vial (ready to use) 2 mL |
| Conjugate Diluent | 1 bottle (ready to use) 12 mL |
| control (low & high) | 2 ml |
| Assay Buffer | 1 bottle (ready to use) 12 mL |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pmol/l) | 0 | 2.6 | 6.6 | 16.5 | 33 | 66 |
| O.D. | 0.16 | 0.25 | 0.36 | 0.63 | 1.06 | 1.82 |
Data Example Images
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Specific Publications For Human Proinsulin ELISA Kit (EK7001)
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Customer Reviews
0 Reviews For Human Proinsulin ELISA Kit
Customer Q&As
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9 Customer Q&As for Human Proinsulin ELISA Kit
Question
I purchased EK7001 Human Proinsulin ELISA kit because they were tested for ELISA. I want to know at what standard were used? Porcine, bovine and human insulin are usually sold but I don
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thank you for asking; the immunogen of EK7001 Human Proinsulin ELISA kit is human insulin. It actually react with human, rat and mouse insulin as well as with proinsulin and intermediates with same affinity. It is logical since the variances between these 3 species are insignificant (different amino acids only in 5 positions). However, it also cross-react with bovine and porcine insulin. All these antigens have been tested. I would rather suggest to use an insulin standard that matches the species of the samples you are testing.
Boster Scientific Support
Answered: 2020-04-09
Question
Hi Boster: I am looking for an Insulin assay, is your product EK7001 Human Proinsulin ELISA kit works in mouse serum?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Indeed, our EK7001 Human Proinsulin ELISA kit is compatible with mouse serum.
Boster Scientific Support
Answered: 2019-12-26
Question
In regards to the EK7001 Human Proinsulin ELISA kit. I want know if it is suitable for staining of proinsulin I or II or both?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Our EK7001 Human Proinsulin ELISA kit reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.
Boster Scientific Support
Answered: 2019-09-27
Question
Dear technical team; In regards to your product EK7001 Human Proinsulin ELISA, I want to know if you have tested it against the insulin analogs, such as humalog (lispro), novolog (aspart), lantus (glarginine). If so, what is the cross-reactivity to these analogs?. Thank you
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The antibody pair that we use in the EK7001 Human Proinsulin ELISA is capture antibody and detection antibody. We have not specifically tested human analogs with EK7001 Human Proinsulin ELISA but based on sequence similarity, the kit recognized these as well. However, we wouldn't guarantee it since it hasn't been specifically tested.
Boster Scientific Support
Answered: 2019-09-03
Question
Hello Support; Your product EK7001 Human Proinsulin ELISA has been tested for western blot. Will you be able to give me a detailed protocol on the western blot for optimal results? I am referring to the polyacrylamide conditions as well.
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
We generally do not recommend exact protocols for performing assays with our products EK7001 Human Proinsulin ELISA. We like to encourage researcher to have a look at our protocols in our website and use it only as guide for their assays. Because every sample is different and may require different conditions. However, this EK7001 Human Proinsulin ELISA antibody was successfully used in mammalian cell culture media in non-reduced conditions. The non-reduced SDS PAGE gel was transferred to nitrocellulose which was then blocked with 4% skim milk for 4 hours. The membrane was washed 3 x 5mins with PBS + 0.05%Tween and then labelled. It is then diluted 1/2000 in PBS for 1 hour with gentle shaking at room temperature. The membrane was washed 3 x 5min with PBS + 0.05% Tween and then labelled with a suitable secondary which was detected by colorimetric method.
Boster Scientific Support
Answered: 2019-08-26
Question
Could you tell me more about the immunogen for this EK7001 Human Proinsulin ELISA kit?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The immunogen of EK7001 Human Proinsulin ELISA kit is purified human insulin. The epitope for this product may be represented by A1, B29 and residues located around them.
Boster Scientific Support
Answered: 2019-08-07
Question
Dear Boster: I am interested in purchasing the EK7001 Human Proinsulin ELISA kit. I want to inquire if this kit is suitable for the measurement of the insulin levels on human cellular extracts/lysates. Thank you.
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The EK7001 Human Proinsulin ELISA kit can detect human insulin through ELISA and you can use cell culture supernatant samples.
Boster Scientific Support
Answered: 2019-02-08
Question
Hi Support I want to use EK7001 Human Proinsulin ELISA human EDTA-plasma samples. Does it work for EDTA plasma?
Verified Customer
Verified customer
Asked: 2015-07-14
Answer
Yes, our EK7001 Human Proinsulin ELISA kit is suitable for EDTA, Heparin and Citrate plasma samples.
Boster Scientific Support
Answered: 2015-07-14
Question
Hello Boster: How do I design EK7001 Human Proinsulin ELISA kit using HRP detection? How do I read the reaction by measuring a particular wavelength?
Verified Customer
Verified customer
Asked: 2013-11-14
Answer
You would need to use a substrate for horseradish peroxidase, such as TMB, to detect the antibody. Kindly see our ELISA protocols on our website for more information about on how to run an ELISA
Boster Scientific Support
Answered: 2013-11-14
