RABBIT MONOCLONAL ANTIBODIES

Rabbit Monoclonal Antibody Advantages And Production

    Browse All Rabbit Monoclonals

    Need to create custom Rabbit monoclonal antibodies ?

    Explore Boster Bio's state of art RABBIT MONOCLONAL ANTIBODY DISCOVERY SERVICE. Our plasma cell discovery (PCD) platform is the best solution for clinical and diagnostic antibody discovery.

    What Are Rabbit Monoclonal Antibodies?

    Monoclonal antibodies are used as primary antibodies in research because of their high specificity for a unique epitope of an antigen, which also leads to minimal lot-to-lot variation and low non-specific cross-reactivity. After the discovery of the hybridoma technique designed by Caesar Millstein and Georges Kohler to produce monoclonal antibodies in 1975, monoclonal antibodies have been conventionally developed in mice for various applications. Over the years, other new techniques have led to the emergence of monoclonal antibodies raised against rabbits, rats, and hamsters. Although mouse monoclonal antibodies have remained as the most commonly used type of monoclonal antibody, a 2015 CiteAb study observed accelerating growth in the number of researchers using rabbit monoclonal antibodies while mouse monoclonals have experienced a steady decrease in the percentage of citations.

    Why is rabbit monoclonal antibodies better ?

    The rabbit immune system can produce greater antibody diversity due to the factors below.

    Somatic Gene Conversion & Somatic Hypermutation

    Somatic Gene Conversion & Somatic Hypermutation

    Somatic gene conversion and hypermutation influence the variable regions of immunoglobulin (Ig) genes, which happen more often in rabbits than rodents. This ultimately contributes to enhancing the rabbit monoclonal antibody repertoire.

    IgG Vs IgM

    IgG Vs IgM

    Rabbits have more IgG as an isotype compared to mice which have a higher ratio of IgM. Because IgG has better specificity than IgM, rabbits have more potential to develop specific antibodies.

    CDRs (Complementarity-Determining Regions)

    CDRs (Complementarity-Determining Regions)

    Since rabbit CDR3 sequences are longer and more heterogeneous, more sequence variation can occur.

    Lower Immunodominance

    Lower Immunodominance

    Immunodominance refers to particular epitopes being more immunogenic than other epitopes of the same antigen, which causes the immune system to mainly generate antibodies against the dominantly immunogenic epitope. The rabbit’s immune system has lower immunodominance than mouse, which allows for more antibody diversity.

    Larger Number Of Lymphocytes

    Larger Number Of Lymphocytes

    Rabbits have larger spleens and other immune organs than mice, which indicates that more lymphocytes (B cells) are available for producing antibodies.

    Extended Period Of Affinity Maturation

    Extended Period Of Affinity Maturation

    After immunization, affinity maturation can persist for a longer period of time for rabbits than mice, resulting in higher affinity and specificity for rabbit monoclonal antibodies.

    Benefits Of Rabbit Monoclonal Antibodies

    Due to the unique features of the rabbit immune system, rabbit monoclonal antibodies are capable of offering the following benefits:

    High Specificity & Affinity

    Rabbit monoclonal antibodies are effective in recognizing minor epitope variations and cleaved versions of a target protein. Furthermore, rabbit monoclonal antibodies can have extremely low equilibrium dissociation constants (KD) in the picomolar range (KD = 10-12M).

    Low Background Noise

    As rabbit monoclonal antibodies only detect a single epitope, they have a low chance of cross-reacting with other proteins. Since rabbit monoclonal antibodies also bind to the target antigen with greater affinity, they prove to have a higher signal-to-noise ratio than mouse monoclonal antibodies.

    Ideal For IHC

    For IHC, antibodies need to be able to identify epitopes in a background with more noise (i.e. FFPE tissues). In this case, rabbit monoclonal antibodies are an excellent choice because they can be highly sensitive while retaining specificity even in strict applications like IHC.

    Good For Post-Translational Modification (PTM) Detection

    The rabbit immune system can produce antibodies against small epitopes existing in small molecules, lipids, and polymers, which permit rabbit monoclonal antibodies to identify subtle changes from PTMs (e.g., phosphorylation, methylation, acetylation, sumoylation, etc.) or single amino acid substitutions.

    Novel Epitope Recognition For Human, Mouse, And Rat Targets

    In comparison to the mouse immune system, the rabbit immune system possesses reduced immunodominance and greater B-cell diversity, which enables wider epitope detection and a higher possibility of discovering unique epitopes when developing rabbit monoclonal antibodies.

    Validated For Flow, IHC, WB, ICC, IF, IP

    Our rabbit monoclonal antibodies have been validated for various applications including WB, IHC, ICC/IF, IP, and flow cytometry as well as common species like human, mouse, and rat.

    Rabbit Monoclonal Vs. Mouse Monoclonal

    Comparison Rabbit Monoclonal Antibodies Mouse Monoclonal Antibodies
    Antigen Detection
    • Better at identifying subtle differences (e.g., epitope variations, like modifications, mutations, conformational changes)
    • Able to detect a wider range of antigens, like small molecules and peptides
    • Identifies multiple epitopes per target antigen
    • Often non-immunogenic for small molecules and peptides
    • Immunodominance limits the number of epitopes recognized
    Affinity
    • 10-100x higher affinity for target antigen than mouse monoclonal antibodies (Picomolar range: 10-12 KD M)
    • Enhanced affinity, including antigens that are poorly immunogenic in mice and humans
    • Lower affinity than rabbit monoclonal antibodies (Nanomolar range: ~10-9 KD M)
    Specificity High Medium or high

    Rabbit Monoclonal Antibody Production

    The general stages of rabbit monoclonal antibody production consist of rabbit immunization, plasma cell isolation, cloning and expression, and screening and selection. The rabbit monoclonal antibodies produced from a single clone are characterized for specific applications (e.g., ELISA, WB, IHC, etc.) and the best clone is chosen for scaling up production and performing purification. Because of the expensive and time-consuming process (usually 4-6 months) of monoclonal antibody production, they are typically more expensive than polyclonal antibodies.

    Immunize rabbits

    Isolate rabbit spleen cells

    Fuse with myeloma partner cells

    Recover hybridoma cells

    Screen for monospecific clones

    Produce best antibodies on small scales

    Characterize antibodies by application

    Scale up production and purification

    Custom Rabbit Monoclonal Antibodies

    Bosterbio provides custom antibody production and development services for rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies. For more details about how our service can help your research, visit our custom rabbit monoclonal antibody production service page to see service information, deliverables, and expected costs. If you have any questions about your custom rabbit monoclonal antibody project, please feel free to contact us to discuss and request a quote.

    Need to create custom Rabbit monoclonal antibodies ?

    Explore Boster Bio's state of art RABBIT MONOCLONAL ANTIBODY DISCOVERY SERVICE. Our plasma cell discovery (PCD) platform is the best solution for clinical and diagnostic antibody discovery.

    Boster Rabbit Monoclonal Antibodies In Research

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    IHC Protocol & Resource Center

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    Western Blotting Protocol & Resource Center

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    Primary Antibodies

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    About Boster Biological Technology

    Here are some quick facts about bosterbio.com

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    Protocol Overview

    Immunohistochemistry (IHC-P)

    • Tissue Preparation
    • Deparaffinization
    • Inactivation
    • Antigen Retrieval
    • Blocking
    • Primary Antibody Incubation
    • Secondary Antibody Incubation
    • Staining
    View IHC-P & IHC-F Protocols

    Western Blot

    • Protein Extraction
    • Protein Quantitation Assay
    • Gel Electrophoresis
    • Protein Transfer
    • Blocking
    • Primary Antibody Incubation
    • Secondary Antibody Incubation
    • Chromogenic or Chemiluminescent Detection
    View WB Protocol

    Flow Cytometry

    • Direct immunostaining of surface antigens
    • Indirect immunostaining of surface antigens
    • General immuno-staining procedure for intracellular antigens
    • Intracellular cytokine/phospho-immunostaining
    • In Vitro Cell Stimulation Reference Table
    • Dye efflux staining
    • DNA content or Cell cycle analysis
    View Flow Cytometry Protocol

    FAQs

    Yes. We have over 29 years of experience in developing antibodies and ELISA kits with our proprietary platform. Our antibodies and ELISA kits are manufactured in-house and rigorously tested to ensure high quality so that our products will work as expected for your experiments.

    Our online datasheets and COAs are not lot-specific. We provide the specific datasheets and COAs when we ship out antibodies. If you would like to request a lot-specific datasheet and/or COA, please contact [email protected] and provide the lot number.

    Q3. Can I get a trial sample of the rabbit monoclonal antibody?

    If available, an option for a 30ug trial sample size will be shown on the product page.

    Q4. Can I get a discount for BosterBio antibodies?

    We generally provide discounts for bulk orders. We also offer a “Buy Primary Antibody, Get Free HRP or Biotin Secondary for Free” promotion.

    Q5. Which types of antibodies does BosterBio provide? How do I choose the right antibody for my experiment?

    We provide primary antibodies and secondary antibodies. Our primary antibodies include rabbit polyclonal antibodies, rabbit monoclonal antibodies, and mouse monoclonal antibodies. We also offer several types of unconjugated and conjugated secondary antibodies. Selecting the right antibody is dependent on the application, specificity, cross-reactivity, species, and immunogen required for your experiment. If you have special conjugation requests or need any technical assistance, please contact [email protected].

    Q6. Can BosterBio antibodies be used for multiple applications?

    Our antibodies can be used for the validated applications listed on the product page. If the antibody does not work for a validated application shown on the product page, please contact [email protected]. Our Boster Guarantee states that if our technicians are unable to make your experiment work, we will offer a refund or replacement.

    Q7. Will the antibody work for an application that is not listed on the product page?

    Although an antibody may work for an application not shown on the product page, it is not covered by our Boster Guarantee. You can contact [email protected] to check if our lab has any data for the requested application.

    Q8. Is the immunogen sequence provided?

    We provide the immunogen sequence for most of our antibodies. If the immunogen sequence is not listed, please contact the [email protected] to check if the information can be shared.

    Q9. What is the antibody clone ID? What is the difference between the clone ID and the lot number?

    The antibody clone ID is a unique identity number assigned to each monoclonal antibody clone. The lot number is the identifier for each batch of antibodies produced.

    Q10. Can BosterBio develop a custom rabbit monoclonal antibody for me if I do not find a suitable rabbit monoclonal antibody from the catalog?

    If you cannot find a suitable rabbit monoclonal antibody from our catalog, please feel free to view details of our custom rabbit monoclonal antibody production service and contact us for a free consultation.

    Q11. If the antibody does not work, can I request a replacement or refund?

    If the antibody does not work for an application we have listed on the product page, it is covered by our Boster Guarantee. You can contact [email protected] for technical troubleshooting. If our technicians are unable to help make your experiment work, we will provide a replacement or refund.

    Q12. What is the recommended concentration I should use for the primary antibody? How do I decide the suitable antibody dilution?

    Recommended dilutions are listed on our product datasheets. However, the end user will need to determine the optimal dilution ratios because of different detection devices, methods, samples, and other factors.

    Q13. Why is the actual band size different from the predicted band size for my western blot?

    Please see this blog for possible reasons. If you have any questions, please contact [email protected].

    Q14. When can I receive the products after I place an order??

    For US customers, the lead time is typically 7-10 business days after you have placed your order. For international customers, please contact your distributor for time estimates. Custom rabbit monoclonal antibodies follow a different timeline. Please visit our custom rabbit monoclonal antibody service page and contact us for more details about the service and production timeline.

    Q15. How do you ship antibodies?

    We ship antibodies through FedEx Priority and provide the tracking number to you. The package will include wet ice.

    Q16. How should I aliquot the antibodies?

    Aliquot volumes are dependent on the amount needed for your experiment. However, we recommend aliquots should 10μL or more. If an aliquot has too little volume, antibody concentration may be affected by liquid evaporation and/or container adsorption.

    Q17. How should I store the antibodies? What is the shelf life of BosterBio's antibodies?

    Our rabbit monoclonal antibodies can be stored at -20°C for one year. After reconstitution, the rabbit monoclonal antibody should be stored at 4°C for one month. If aliquotted, the antibody can be stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

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    Primary Antibodies

    Primary and Secondary Antibodies

    Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

    ELISA Kits

    ELISA Kits

    More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine® ELISA kits (sandwich ELISA) and EZ-Set® ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

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    Recombinant Proteins

    Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

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    Reagents

    Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.